PXD019051

PXD019051 is an original dataset announced via ProteomeXchange.

Title	Acute effects of Salbutamol in Mouse Kidney Distal Convoluted Tubules (mpkDCT) Cells
Description	The sympathetic nervous system (SNS) plays a central role in blood pressure regulation. Recent studies have shown that cells of the distal convoluted tubule (DCT) can be stimulated directly via the beta-adrenergic receptor resulting in activation of the NaCl cotransporter NCC. Whether these effects are mediated by the beta1- or beta2-adrenergic receptor is unclear, and the acute signaling cascades rapidly activated by beta-adrenergic receptor stimulation in the DCT are unknown. Here in this study, we aim to identify the rapid salbutamol-mediated (beta2-adrenergic receptor) signaling in the DCT by looking at global protein phosphorylation changes in the mpkDCT cells.
HostingRepository	PRIDE
AnnounceDate	2021-05-18
AnnouncementXML	Submission_2021-05-17_22:00:37.446.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lei Cheng
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-07 22:46:20	ID requested
1	2021-05-17 06:21:40	announced
⏵ 2	2021-05-17 22:00:38	announced	2021-05-18: Updated project metadata.

Poulsen SB, Cheng L, Penton D, Kortenoeven MLA, Matchkov VV, Loffing J, Little R, Murali SK, Fenton RA, 2-adrenergic receptor agonist salbutamol increases blood pressure. Kidney Int, 100(2):321-335(2021) [pubmed]

10.1016/J.KINT.2021.04.021;

submitter keyword: Beta2-adrenergic receptor, salbutamol, hypertention, DCT

Robert A. Fenton
contact affiliation	Institute of biomedicine, Aarhus University
contact email	robert.a.fenton@biomed.au.dk
lab head
Lei Cheng
contact affiliation	Institute of biomedicine, AU
contact email	lche@biomed.au.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD019051

PRIDE project URI

• PRIDE
  1. PXD019051
     1. Label: PRIDE project
     2. Name: Acute effects of Salbutamol in Mouse Kidney Distal Convoluted Tubules (mpkDCT) Cells