PXD019040

PXD019040 is an original dataset announced via ProteomeXchange.

Title	Identification of RNG140 (caprin2)-associated proteins in CHO cells
Description	Regulation of gene expression at the translational level is key to determining cell fate and function. An RNA-binding protein RNG140 (caprin2) plays a role in cell differentiation such as eye lens development and has been reported to function in translational regulation. To identify RNG140-associated proteins, we generated CHO cells stably expressing GFP and RNG140-GFP, and performed immunoprecipitation (IP) with GFP antibody from those cells followed by mass spectrometry. We identified about 400 proteins specifically co-immunoprecipitated with RNG140-GFP but not with GFP. Gene ontology (GO) enrichment analysis revealed that the eIF3 subunit proteins and small ribosomal subunit proteins were significantly enriched in the RNG140-associated complex.
HostingRepository	PRIDE
AnnounceDate	2020-08-17
AnnouncementXML	Submission_2020-08-16_22:35:31.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kaori Nakazawa
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-07 06:29:48	ID requested
⏵ 1	2020-08-16 22:35:32	announced

Dataset with its publication pending

submitter keyword: CHO, RNA-binding protein, RNG140, caprin2, LC-MS

Nobuyuki Shiina
contact affiliation	Laboratory of Neuronal Cell Biology, National Institute for Basic Biology, Okazaki, Aichi
contact email	nshiina@nibb.ac.jp
lab head
Kaori Nakazawa
contact affiliation	National Institute for Basic Biology
contact email	katayama@nibb.ac.jp
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019040
     1. Label: PRIDE project
     2. Name: Identification of RNG140 (caprin2)-associated proteins in CHO cells