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PXD019023

PXD019023 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleStudy of Brucella abortus / Agrobacterium tumefaciens outer membrane proteins covalently bound to peptidoglycan
DescriptionGram negative bacteria are surrounded by the cell envelope, a structure comprised of an outer membrane (OM) and an inner membrane (IM). These two membranes delimit the periplasmic space, a compartment containing the peptidoglycan (PG), a giant polymer surrounding the cell and functioning as an extracytoplasmic skeleton. In the model organism Escherichia coli, covalently anchoring the OM to the peptidoglycan is crucial for envelope integrity. When attachment is prevented, the OM forms blebs and detaches from the cell body. Intriguingly, the only bacterial protein known to covalently attach the OM to the PG (the Braun lipoprotein or Lpp) is present in a small number of γ-proteobacteria, raising the question of OM-PG attachment in other species. Here, we show that in -proteobacteria Brucella abortus and Agrobacterium tumefaciens, the OM is attached to the peptidoglycan via the formation of covalent crosslinks between the N-terminus of integral OM proteins (OMPs; including porins) and peptide stems of peptidoglycan.
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_10:41:42.072.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD019023
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMarc Dieu
SpeciesList scientific name: Brucella abortus (strain 2308); NCBI TaxID: 359391; scientific name: Agrobacterium fabrum str. C58; NCBI TaxID: 176299;
ModificationListAmmonia-loss; Deamidated; Oxidation; Carbamidomethyl; monohydroxylated residue; iodoacetamide derivatized residue; Gln->pyro-Glu; deamidated residue
InstrumentmaXis
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-05-06 19:13:37ID requested
12024-10-07 10:41:42announced
Publication List
10.6019/PXD019023;
Keyword List
submitter keyword: maxis impact, Agrobacterium tumefaciens, peptidoglycan,Brucella abortus, OMP
Contact List
Prof Xavier De Bolle
contact affiliationResearch Unit in Microorganisms Biology (URBM), Namur Research Institute for Life Sciences (NARILIS), UNamur, rue deBruxelles 61, 5000 Namur, Belgium.
contact emailxavier.debolle@unamur.be
lab head
Marc Dieu
contact affiliationUniversity of Namur - MaSUN
contact emailmarc.dieu@unamur.be
dataset submitter
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Dataset FTP location
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