PXD019023 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Study of Brucella abortus / Agrobacterium tumefaciens outer membrane proteins covalently bound to peptidoglycan |
Description | Gram negative bacteria are surrounded by the cell envelope, a structure comprised of an outer membrane (OM) and an inner membrane (IM). These two membranes delimit the periplasmic space, a compartment containing the peptidoglycan (PG), a giant polymer surrounding the cell and functioning as an extracytoplasmic skeleton. In the model organism Escherichia coli, covalently anchoring the OM to the peptidoglycan is crucial for envelope integrity. When attachment is prevented, the OM forms blebs and detaches from the cell body. Intriguingly, the only bacterial protein known to covalently attach the OM to the PG (the Braun lipoprotein or Lpp) is present in a small number of γ-proteobacteria, raising the question of OM-PG attachment in other species. Here, we show that in -proteobacteria Brucella abortus and Agrobacterium tumefaciens, the OM is attached to the peptidoglycan via the formation of covalent crosslinks between the N-terminus of integral OM proteins (OMPs; including porins) and peptide stems of peptidoglycan. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-07 |
AnnouncementXML | Submission_2024-10-07_10:41:42.072.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD019023 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Marc Dieu |
SpeciesList | scientific name: Brucella abortus (strain 2308); NCBI TaxID: 359391; scientific name: Agrobacterium fabrum str. C58; NCBI TaxID: 176299; |
ModificationList | Ammonia-loss; Deamidated; Oxidation; Carbamidomethyl; monohydroxylated residue; iodoacetamide derivatized residue; Gln->pyro-Glu; deamidated residue |
Instrument | maXis |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-05-06 19:13:37 | ID requested | |
⏵ 1 | 2024-10-07 10:41:42 | announced | |
Publication List
Keyword List
submitter keyword: maxis impact, Agrobacterium tumefaciens, peptidoglycan,Brucella abortus, OMP |
Contact List
Prof Xavier De Bolle |
contact affiliation | Research Unit in Microorganisms Biology (URBM), Namur Research Institute for Life Sciences (NARILIS), UNamur, rue deBruxelles 61, 5000 Namur, Belgium. |
contact email | xavier.debolle@unamur.be |
lab head | |
Marc Dieu |
contact affiliation | University of Namur - MaSUN |
contact email | marc.dieu@unamur.be |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD019023 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019023
- Label: PRIDE project
- Name: Study of Brucella abortus / Agrobacterium tumefaciens outer membrane proteins covalently bound to peptidoglycan