PXD019023

PXD019023 is an original dataset announced via ProteomeXchange.

Title	Study of Brucella abortus / Agrobacterium tumefaciens outer membrane proteins covalently bound to peptidoglycan
Description	Gram negative bacteria are surrounded by the cell envelope, a structure comprised of an outer membrane (OM) and an inner membrane (IM). These two membranes delimit the periplasmic space, a compartment containing the peptidoglycan (PG), a giant polymer surrounding the cell and functioning as an extracytoplasmic skeleton. In the model organism Escherichia coli, covalently anchoring the OM to the peptidoglycan is crucial for envelope integrity. When attachment is prevented, the OM forms blebs and detaches from the cell body. Intriguingly, the only bacterial protein known to covalently attach the OM to the PG (the Braun lipoprotein or Lpp) is present in a small number of γ-proteobacteria, raising the question of OM-PG attachment in other species. Here, we show that in -proteobacteria Brucella abortus and Agrobacterium tumefaciens, the OM is attached to the peptidoglycan via the formation of covalent crosslinks between the N-terminus of integral OM proteins (OMPs; including porins) and peptide stems of peptidoglycan.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:41:42.072.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019023
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Marc Dieu
SpeciesList	scientific name: Brucella abortus (strain 2308); NCBI TaxID: 359391; scientific name: Agrobacterium fabrum str. C58; NCBI TaxID: 176299;
ModificationList	Ammonia-loss; Deamidated; Oxidation; Carbamidomethyl; monohydroxylated residue; iodoacetamide derivatized residue; Gln->pyro-Glu; deamidated residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-06 19:13:37	ID requested
⏵ 1	2024-10-07 10:41:42	announced

10.6019/PXD019023;

submitter keyword: maxis impact, Agrobacterium tumefaciens, peptidoglycan,Brucella abortus, OMP

Prof Xavier De Bolle
contact affiliation	Research Unit in Microorganisms Biology (URBM), Namur Research Institute for Life Sciences (NARILIS), UNamur, rue deBruxelles 61, 5000 Namur, Belgium.
contact email	xavier.debolle@unamur.be
lab head
Marc Dieu
contact affiliation	University of Namur - MaSUN
contact email	marc.dieu@unamur.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD019023

PRIDE project URI

• PRIDE
  1. PXD019023
     1. Label: PRIDE project
     2. Name: Study of Brucella abortus / Agrobacterium tumefaciens outer membrane proteins covalently bound to peptidoglycan