PXD019020

PXD019020 is an original dataset announced via ProteomeXchange.

Title	Systematic analysis of IL-17 receptor signalosome reveals a robust regulatory feedback loop
Description	IL-17 mediates immune protection from fungi and bacteria as well as it promotes autoimmune pathologies. However, the regulation of the signal transduction from the IL-17 receptor (IL-17R) remained elusive. We developed a novel mass spectrometry-based approach to identify components of the IL-17R complex followed by analysis of their roles using reverse genetics. Besides the identification of LUBAC as an important signal transducing component of IL-17R, we established that IL-17 signaling is regulated by a robust negative feedback loop mediated by TBK1 and IKKε. These kinases terminate IL-17 signaling by phosphorylating the adaptor ACT1 leading to the release of the essential ubiquitin ligase TRAF6 from the complex. NEMO recruits both kinases to the IL-17R complex, documenting that NEMO has an unprecedented negative function in IL-17 signaling, distinct from its role in NF-κB activation. Our study provides a comprehensive view of the molecular events of the IL-17 signal transduction and its regulation.
HostingRepository	PRIDE
AnnounceDate	2020-07-15
AnnouncementXML	Submission_2020-07-15_07:04:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Karel Harant
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	carbamoylated residue
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-06 18:53:37	ID requested
⏵ 1	2020-07-15 07:04:49	announced

Dataset with its publication pending

submitter keyword: IL-17, TRAF6, TBK1, IKKε, LUBAC

Peter Draber
contact affiliation	Laboratory of Immunity & Cell Communication, BIOCEV, First Faculty of Medicine, Charles University, Vestec, Czech Republic
contact email	peter.draber@lf1.cuni.cz
lab head
Karel Harant
contact affiliation	Charles University
contact email	harant@natur.cuni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD019020

PRIDE project URI

• PRIDE
  1. PXD019020
     1. Label: PRIDE project
     2. Name: Systematic analysis of IL-17 receptor signalosome reveals a robust regulatory feedback loop