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PXD019019

PXD019019 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNsiR6/NblD is a small protein factor involved in the degradation of phycobilisomes under N-limiting conditions
DescriptionSmall proteins are an under investigated class of gene products in all domains of life. Here we describe the role of NsiR6/NblD, a cysteine-rich 66 amino acid small protein in the acclimation response of cyanobacteria to nitrogen starvation. Phycobilisomes, the macromolecular pigment-protein complexes for photosynthetic light harvesting, are rapidly degraded upon nitrogen depletion. Deletion of nblD in Synechocystis sp. strain PCC 6803 prevents this degradation, indicated by the non-bleaching (nbl) phenotype. Complementation by a plasmid-localized gene copy fully restored the phenotype of the wild type, while overexpression of NblD under nitrogen-replete conditions did not lead to any phenotypical effect, different from the unrelated proteolysis adaptors NblA1 and NblA2, which can trigger phycobilisome degradation ectopically. However, transcriptome analysis revealed that nitrogen starvation induced nblA1/2 transcription in the ΔnblD strain, which excluded the possibility that the nbl phenotype was due to a possible NblD function as transcriptional co-regulator. In contrast, fractionation experiments indicated the presence of NblD in the phycobilisome fraction. Pull-down experiments with NblD fused to a triple FLAG tag identified the α and β phycocyanin subunits as the only two co-purifying proteins. Homologs of NblD exist in all cyanobacteria that use phycobilisomes but not in the genera Prochlorococcus and Acaryochloris which use alternative light-harvesting mechanisms. These data suggest that NblD plays a crucial role in the coordinated dismantling of phycobilisomes when nitrogen becomes limiting.
HostingRepositoryPRIDE
AnnounceDate2021-03-04
AnnouncementXMLSubmission_2021-03-04_14:36:01.597.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterOliver Schilling
SpeciesList scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-05-06 18:53:20ID requested
12021-03-04 14:36:01announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Small proteins, NsiR6/NblD, Cyanobacteria, Co-IP MS
Contact List
Oliver Schilling
contact affiliationInstitute for Surgical Pathology, Medical Center – University of Freiburg, Faculty of Medicine, University of Freiburg, Germany
contact emailoliver.schilling@mol-med.uni-freiburg.de
lab head
Oliver Schilling
contact affiliationUniversity of Freiburg
contact emailoliver.schilling@mol-med.uni-freiburg.de
dataset submitter
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