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PXD019004

PXD019004 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleReliable identification of protein-protein interactions by crosslinking mass spectrometry_Protein coelution
DescriptionQuantitative proteomics dataset on soluble, SEC-fractionated high-molecular weight proteome of E. coli for protein coelution analysis. This data was used to identify proteins for a subsequent crosslinking mass spectrometry search (DB creation) and to correlate protein elution behaviour to validate/falsify potential PPIs from the CLMS-based approach. Cleared E. coli lysate was fractionated in the range of 3 MDa to 150 kDa (44 fractions). The proteins in each fraction were precipitated, derivatized and proteolysed in-solution. Then, each fraction was acquired via LC-MS on Q Exactive HF and the raw data searched with MaxQuant with iBAQ quantitation enabled.
HostingRepositoryjPOST
AnnounceDate2021-04-14
AnnouncementXMLSubmission_2022-09-18_03:46:25.711.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLudwig Sinn
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListS-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
Instrumentinstrument
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-05-05 13:23:17ID requested
12021-04-14 01:41:18announced
22022-09-18 03:46:26announced2022-09-18: Updated FTP location.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: crosslinking, mass spectrometry, FDR, protein-protein interaction, E. coli
Contact List
Prof. Dr. Juri Rappsilber
lab head
Ludwig Sinn
contact affiliationTechnische Universit├Ąt Berlin, Bioanalytics, Prof. Juri Rappsilber
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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