PXD018993

PXD018993 is an original dataset announced via ProteomeXchange.

Title	Comprehensive protein profiling of tomato phloem exudates during drought stress and recovery
Description	Phloem is the primary conduit through which photosynthates, hormones, and other biologically important molecules are distributed from aerial plant organs downward and throughout the plant. Increasing evidence suggests that phloem contents are diverse and play a critical role in biotic and abiotic stress adaptation. Drought causes the greatest decreases in agricultural crop productivity among all biotic and abiotic stresses, and the link between water deficiency and phloem protein contents is relatively unexplored. Here we employed the EDTA-facilitated phloem exudate collection method from Solanum lycopersicum leaves during a period of drought stress and recovery. Our analysis resulted in the confident identification and quantification of 2,558 proteins. Comparing our data with previous findings confirms that our exudate collection strategy enriched for known phloem proteins. Independent of drought, enrichment analysis of the total phloem exudate protein profiles from all samples suggest that the exchange of proteins into the phloem is more complex than previously thought, including additional protein chaperone systems, branched-chain amino acid synthesis proteins, trehalose metabolism, and RNA silencing proteins. During the experiment we observed 169 proteins whose abundance changed significantly within the phloem sap, the majority of which were impacted specifically in response to drought. Among these were proteins involved in lipid metabolism, chaperone-mediated protein folding, carboxylic acid metabolism, abscisic acid signaling, cytokinin biosynthesis, and amino acid metabolism are significantly upregulated during drought. Conversely, proteins involved in lipid signaling, sphingolipid metabolism, cell wall organization, carbohydrate metabolism, and a mitogen-activated protein kinase are downregulated in response to drought. Many of these observations are consistent with previous literature findings at the whole plant level but have not been localized to the vasculature in tomato, suggesting phloem plays a critical role in adaptation to drought stress in tomato .
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:51:03.768.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aaron Ogden
SpeciesList	scientific name: Solanum lycopersicum; NCBI TaxID: 4081;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-05 01:20:11	ID requested
1	2020-06-26 05:31:59	announced
2	2020-06-30 04:03:45	announced	2020-06-30: Updated publication reference for PubMed record(s): 32586033. 2020-06-30: Updated publication reference for DOI(s): 10.3390/ijms21124461.
⏵ 3	2023-11-14 08:51:04	announced	2023-11-14: Updated project metadata.

Ogden AJ, Bhatt JJ, Brewer HM, Kintigh J, Kariuki SM, Rudrabhatla S, Adkins JN, Curtis WR, Phloem Exudate Protein Profiles during Drought and Recovery Reveal Abiotic Stress Responses in Tomato Vasculature. Int J Mol Sci, 21(12):(2020) [pubmed]

submitter keyword: exudate, tomato, abiotic stress,phloem, phloem exudate, drought

Josh Adkins
contact affiliation	Pacific Northwest National Labs
contact email	joshua.adkins@pnnl.gov
lab head
Aaron Ogden
contact affiliation	Postdoctoral researcher
contact email	Aaron.ogden@pnnl.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD018993

PRIDE project URI

• PRIDE
  1. PXD018993
     1. Label: PRIDE project
     2. Name: Comprehensive protein profiling of tomato phloem exudates during drought stress and recovery