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PXD018993

PXD018993 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComprehensive protein profiling of tomato phloem exudates during drought stress and recovery
DescriptionPhloem is the primary conduit through which photosynthates, hormones, and other biologically important molecules are distributed from aerial plant organs downward and throughout the plant. Increasing evidence suggests that phloem contents are diverse and play a critical role in biotic and abiotic stress adaptation. Drought causes the greatest decreases in agricultural crop productivity among all biotic and abiotic stresses, and the link between water deficiency and phloem protein contents is relatively unexplored. Here we employed the EDTA-facilitated phloem exudate collection method from Solanum lycopersicum leaves during a period of drought stress and recovery. Our analysis resulted in the confident identification and quantification of 2,558 proteins. Comparing our data with previous findings confirms that our exudate collection strategy enriched for known phloem proteins. Independent of drought, enrichment analysis of the total phloem exudate protein profiles from all samples suggest that the exchange of proteins into the phloem is more complex than previously thought, including additional protein chaperone systems, branched-chain amino acid synthesis proteins, trehalose metabolism, and RNA silencing proteins. During the experiment we observed 169 proteins whose abundance changed significantly within the phloem sap, the majority of which were impacted specifically in response to drought. Among these were proteins involved in lipid metabolism, chaperone-mediated protein folding, carboxylic acid metabolism, abscisic acid signaling, cytokinin biosynthesis, and amino acid metabolism are significantly upregulated during drought. Conversely, proteins involved in lipid signaling, sphingolipid metabolism, cell wall organization, carbohydrate metabolism, and a mitogen-activated protein kinase are downregulated in response to drought. Many of these observations are consistent with previous literature findings at the whole plant level but have not been localized to the vasculature in tomato, suggesting phloem plays a critical role in adaptation to drought stress in tomato .
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:51:03.768.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAaron Ogden
SpeciesList scientific name: Solanum lycopersicum; NCBI TaxID: 4081;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-05-05 01:20:11ID requested
12020-06-26 05:31:59announced
22020-06-30 04:03:45announced2020-06-30: Updated publication reference for PubMed record(s): 32586033.
2020-06-30: Updated publication reference for DOI(s): 10.3390/ijms21124461.
32023-11-14 08:51:04announced2023-11-14: Updated project metadata.
Publication List
Ogden AJ, Bhatt JJ, Brewer HM, Kintigh J, Kariuki SM, Rudrabhatla S, Adkins JN, Curtis WR, Phloem Exudate Protein Profiles during Drought and Recovery Reveal Abiotic Stress Responses in Tomato Vasculature. Int J Mol Sci, 21(12):(2020) [pubmed]
Keyword List
submitter keyword: exudate, tomato, abiotic stress,phloem, phloem exudate, drought
Contact List
Josh Adkins
contact affiliationPacific Northwest National Labs
contact emailjoshua.adkins@pnnl.gov
lab head
Aaron Ogden
contact affiliationPostdoctoral researcher
contact emailAaron.ogden@pnnl.gov
dataset submitter
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Dataset FTP location
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PRIDE project URI
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