PXD018982

PXD018982 is an original dataset announced via ProteomeXchange.

Title	Cytokine-enhanced cytolytic activity of Exosomes from NK Cells
Description	Natural killer (NK) cells are a type of innate lymphocytes that play key roles in immune surveillance against tumors and viral infection. NK cells distinguish abnormal cells from healthy cells by cell-cell interaction with cell surface proteins and then attack target cells via multiple mechanisms involving TRAIL, Fas Ligand, cytokine secretion, perforin, and granzymes. In addition, extracellular vesicles (EVs), including exosomes derived from NK cells (NK-EVs), possess cytotoxic capacity against tumor cells, but their characteristics and regulation by cytokines remain unknown. Here, we report that EVs derived from human NK-92 cells stimulated with IL-15 + IL-21 show enhanced cytotoxic capacity against tumor cells in a granzyme B independent manner. In addition, small RNA-seq and mass spectrometry analyses indicate that miRNA and protein profiles in EVs are altered by cytokine stimulation. We also show NK-EVs are taken up by target cells via macropinocytosis. Collectively, our findings reveal novel characteristics of NK-EVs and the mechanism of their incorporation into target cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-11
AnnouncementXML	Submission_2024-10-11_03:15:35.377.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD018982
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lisa Jenkins
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Acetyl; Carbamidomethyl
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-04 17:51:51	ID requested
⏵ 1	2024-10-11 03:15:36	announced

10.1038/s41417-021-00352-2;

Enomoto Y, Li P, Jenkins LM, Anastasakis D, Lyons GC, Hafner M, Leonard WJ, Cytokine-enhanced cytolytic activity of exosomes from NK Cells. Cancer Gene Ther, 29(6):734-749(2022) [pubmed]

10.6019/PXD018982;

submitter keyword: Exosome, Cytokine, NK Cells

Lisa M. Jenkins
contact affiliation	Laboratory of Cell Biology, National Cancer Institute, NIH United States
contact email	lisa.jenkins@nih.gov
lab head
Lisa Jenkins
contact affiliation	National Cancer Institute, NIH
contact email	lisa.jenkins@nih.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD018982

PRIDE project URI

• PRIDE
  1. PXD018982
     1. Label: PRIDE project
     2. Name: Cytokine-enhanced cytolytic activity of Exosomes from NK Cells