PXD018982
PXD018982 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cytokine-enhanced cytolytic activity of Exosomes from NK Cells |
Description | Natural killer (NK) cells are a type of innate lymphocytes that play key roles in immune surveillance against tumors and viral infection. NK cells distinguish abnormal cells from healthy cells by cell-cell interaction with cell surface proteins and then attack target cells via multiple mechanisms involving TRAIL, Fas Ligand, cytokine secretion, perforin, and granzymes. In addition, extracellular vesicles (EVs), including exosomes derived from NK cells (NK-EVs), possess cytotoxic capacity against tumor cells, but their characteristics and regulation by cytokines remain unknown. Here, we report that EVs derived from human NK-92 cells stimulated with IL-15 + IL-21 show enhanced cytotoxic capacity against tumor cells in a granzyme B independent manner. In addition, small RNA-seq and mass spectrometry analyses indicate that miRNA and protein profiles in EVs are altered by cytokine stimulation. We also show NK-EVs are taken up by target cells via macropinocytosis. Collectively, our findings reveal novel characteristics of NK-EVs and the mechanism of their incorporation into target cells. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-11 |
AnnouncementXML | Submission_2024-10-11_03:15:35.377.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD018982 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Lisa Jenkins |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Oxidation; Acetyl; Carbamidomethyl |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-05-04 17:51:51 | ID requested | |
⏵ 1 | 2024-10-11 03:15:36 | announced |
Publication List
10.1038/s41417-021-00352-2; |
Enomoto Y, Li P, Jenkins LM, Anastasakis D, Lyons GC, Hafner M, Leonard WJ, Cytokine-enhanced cytolytic activity of exosomes from NK Cells. Cancer Gene Ther, 29(6):734-749(2022) [pubmed] |
10.6019/PXD018982; |
Keyword List
submitter keyword: Exosome, Cytokine, NK Cells |
Contact List
Lisa M. Jenkins | |
---|---|
contact affiliation | Laboratory of Cell Biology, National Cancer Institute, NIH United States |
contact email | lisa.jenkins@nih.gov |
lab head | |
Lisa Jenkins | |
contact affiliation | National Cancer Institute, NIH |
contact email | lisa.jenkins@nih.gov |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD018982 |
PRIDE project URI |
Repository Record List
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