PXD018981

PXD018981 is an original dataset announced via ProteomeXchange.

Title	Structure of the full kinetoplastids mitoribosome and insight on its large subunit maturation
Description	Kinetoplastids are unicellular eukaryotic parasites responsible for human pathologies such as Chagas disease, sleeping sickness or Leishmaniasis1. They possess a single large mitochondrion, essential for the parasite survival2. In kinetoplastids mitochondrion, most of the molecular machineries and gene expression processes have significantly diverged and specialized, with the most extreme case being their mitochondrial ribosomes3. These large complexes are in charge of translating the few essential mRNAs encoded by mitochondrial genomes4,5. Structural studies performed in Trypanosoma brucei already highlighted the numerous peculiarities of these mitoribosomes and the maturation of their small subunit3,6. However, several important aspects mainly related to the large subunit remain elusive, such as the structure and maturation of its ribosomal RNA3. Here, we present a cryo-electron microscopy study of the protozoans Leishmania tarentolae and Trypanosoma cruzi mitoribosomes. For both species, we obtained the structure of their mature mitoribosomes, complete rRNA of the large subunit as well as previously unidentified ribosomal proteins. Most importantly, we introduce the structure of an LSU assembly intermediate in presence of 16 identified maturation factors. These maturation factors act both on the intersubunit and solvent sides of the LSU, where they refold and chemically modify the rRNA and prevent early translation before full maturation of the LSU.
HostingRepository	PRIDE
AnnounceDate	2021-04-29
AnnouncementXML	Submission_2021-04-29_08:11:46.694.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD018981
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lauriane Kuhn
SpeciesList	scientific name: Trypanosoma cruzi cruzi; NCBI TaxID: 85057; scientific name: Leishmania tarentolae; NCBI TaxID: 5689;
ModificationList	Phospho; Oxidation; Acetyl; Sulfo; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-04 17:51:23	ID requested
⏵ 1	2021-04-29 08:11:47	announced

Dataset with its publication pending

submitter keyword: mitochondria, ribosome, mitoribosome, kinetoplastids, trypanosoma, leishmania

Florent Waltz
contact affiliation	Institut Européen de Chimie et Biologie (IECB), U1212 Inserm, Université de Bordeaux, 2 rue R. Escarpit, F-33600 Pessac, France
contact email	waltz.florent@gmail.com
lab head
Lauriane Kuhn
contact affiliation	CNRS, IBMC, FRC1589
contact email	l.kuhn@ibmc-cnrs.unistra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018981
     1. Label: PRIDE project
     2. Name: Structure of the full kinetoplastids mitoribosome and insight on its large subunit maturation