PXD018978

PXD018978 is an original dataset announced via ProteomeXchange.

Title	Investigating the protein-protein interactions of EccA3 in Mycobacterium smegmatis
Description	Mycobacterium smegmatis encodes three ESX secretion systems, ESX-1, -3 and -4. Previous research has suggested localisation of the ESX-1 secretion system at the growing polar region of M. smegmatis. In a seperate study, we demonstrated unipolar localisation of EccA3 from ESX-3 at the growing polar region of M. smegmatis. Here we demonstrate that affinity purification of N-terminally FLAG-tagged MSMEG_0615 (EccA3) followed by mass spectrometry resulted in the co-purification proteins previously identified in the membrane fractions of M. tuberculosis. Furthermore, we demonstrate that EccA3 co-purified with proteins known to localise at the growing polar region of asymmetrically growing M. smegmatis, including Wag31 and CwsA.
HostingRepository	PRIDE
AnnounceDate	2022-06-09
AnnouncementXML	Submission_2022-06-09_02:22:57.173.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nastassja Kriel
SpeciesList	scientific name: Mycolicibacterium smegmatis MC2 155; NCBI TaxID: 246196;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-04 16:27:39	ID requested
⏵ 1	2022-06-09 02:22:58	announced

Kriel NL, Newton-Foot M, Bennion OT, Aldridge BB, Mehaffy C, Belisle JT, Walzl G, Warren RM, Sampson SL, Gey van Pittius NC, at the growing pole in Mycobacterium smegmatis. BMC Microbiol, 22(1):140(2022) [pubmed]

submitter keyword: Mycobacterium smegmatis, AP-MS, ESX, polar localisation

Samantha Sampson
contact affiliation	Department of Biomedical Sciences, Division of Molecular Biology and Human Genetics, Stellenbosch University, Cape Town, South Africa.
contact email	ssampson@sun.ac.za
lab head
Nastassja Kriel
contact affiliation	Stellenbosch Univeristy
contact email	nastassja@sun.ac.za
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD018978

PRIDE project URI

• PRIDE
  1. PXD018978
     1. Label: PRIDE project
     2. Name: Investigating the protein-protein interactions of EccA3 in Mycobacterium smegmatis