PXD018976 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mapping specificity, entropy, allosteric changes and substrates in blood coagulation proteases by a high-throughput protease screen |
Description | Proteases are crucial physiologic regulators of protein structure and function. While proteomic methods contributed extensively to protease characterization efforts, technical challenges remain in terms of throughput, scalability and large datasets of protease cleavages remain scarce. Here, we describe a high-throughput protease screen (HTPS) which allows simultaneous characterization of multiple proteases under various conditions on a microscale 96FASP format. After benchmarking the performance with Trypsin, LysC, GluC, AspN, Chymotrypsin, MMP-2 and MMP-3, we applied it to profile proteases of the blood coagulation cascade (Factors VIIa, IXa, Xa, XIa, Thrombin α, β and γ, Plasmin and Protein C). The large dataset enabled us to map activity, specificity, cleave entropy, allosteric changes of blood cascade proteases induced by Na+ and to predict potential substrates in a data-driven way. |
HostingRepository | PRIDE |
AnnounceDate | 2021-02-05 |
AnnouncementXML | Submission_2021-04-06_02:42:43.057.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Matej Vizovisek |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monoacetylated residue; monohydroxylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-05-04 16:26:34 | ID requested | |
1 | 2021-02-04 22:32:23 | announced | |
⏵ 2 | 2021-04-06 02:42:43 | announced | 2021-04-06: Updated publication reference for PubMed record(s): 33727531. |
Publication List
Uliana F, Vizovi, š, ek M, Acquasaliente L, Ciuffa R, Fossati A, Frommelt F, Goetze S, Wollscheid B, Gstaiger M, De Filippis V, Auf dem Keller U, Aebersold R, Mapping specificity, cleavage entropy, allosteric changes and substrates of blood proteases in a high-throughput screen. Nat Commun, 12(1):1693(2021) [pubmed] |
Keyword List
submitter keyword: blood coagulation proteases, specificity, entropy, allostery, substrates, high-throughput protease screen |
Contact List
Ruedi Aebersold |
contact affiliation | Department of Biology, Institute of Molecular Systems Biology, ETH Zürich, Zürich, Switzerland. Faculty of Science, University of Zürich, Zürich, Switzerland |
contact email | aebersold@imsb.biol.ethz.ch |
lab head | |
Matej Vizovisek |
contact affiliation | ETH Zurich, Inst. f. Molekulare Systembiologie Otto-Stern-Weg 3 8093 Zürich Switzerland |
contact email | vizovisek@imsb.biol.ethz.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018976
- Label: PRIDE project
- Name: Mapping specificity, entropy, allosteric changes and substrates in blood coagulation proteases by a high-throughput protease screen