PXD018946

PXD018946 is an original dataset announced via ProteomeXchange.

Title	BMAL1 associates with NOP58 in the nucleolus and contributes to pre-rRNA processing
Description	The transcription factor BMAL1 is a core element of the circadian clock that contributes to cyclic control of genes transcribed by RNA polymerase II. By using biochemical cellular fractionation and immunofluorescence analyses we reveal a previously uncharacterized nucleolar localization for BMAL1. We used an unbiased approach to determine the BMAL1 interactome by mass spectrometry and identified NOP58 as a prominent nucleolar interactor. NOP58, a core component of the box C/D small nucleolar ribonucleoprotein complex, associates with Snord118 to control specific pre-ribosomal RNA (rRNA) processing steps. These results suggest a non-canonical role of BMAL1 in rRNA regulation. Indeed, we show that BMAL1 controls NOP58-associated Snord118 nucleolar levels and cleavage of unique pre-rRNA intermediates. Our findings identify an unsuspected function of BMAL1 in the nucleolus that appears distinct from its canonical role in the circadian clock system
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:11:20.120.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-01 06:01:47	ID requested
1	2020-08-25 06:29:26	announced
⏵ 2	2024-10-22 05:11:20	announced	2024-10-22: Updated project metadata.

Cervantes M, Forn, é I, Ranjit S, Gratton E, Imhof A, Sassone-Corsi P, BMAL1 Associates with NOP58 in the Nucleolus and Contributes to Pre-rRNA Processing. iScience, 23(6):101151(2020) [pubmed]

10.1016/j.isci.2020.101151;

submitter keyword: BMAL1 interactome, nucleolus, circadian clock, ribosome biogenesis

Paolo Sassone-Corsi
contact affiliation	Center for Epigenetics and Metabolism, U1233 INSERM, Department of Biological Chemistry, University of California, Irvine, 92697 California.
contact email	psc@uci.edu
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD018946

PRIDE project URI

• PRIDE
  1. PXD018946
     1. Label: PRIDE project
     2. Name: BMAL1 associates with NOP58 in the nucleolus and contributes to pre-rRNA processing