PXD018945

PXD018945 is an original dataset announced via ProteomeXchange.

Title	Nuclear moonlighting of cytosolic glyceraldehyde-3-phosphate dehydrogenase regulates Arabidopsis response to heat stress
Description	Various stress conditions induce the nuclear translocation of cytosolic glyceraldehyde-3-phosphate dehydrogenase (GAPC), but its nuclear function in plant stress responses remains elusive. Here we show that GAPC interacts with a transcription factor to increase the expression of heat-inducible genes and heat tolerance in Arabidopsis. GAPC accumulates in the nucleus in a heat stress-dependent manner. Screening of Arabidopsis transcription factors identifies nuclear factor Y subunit C10 (NF-YC10) as a GAPC-binding protein. Heat tolerance of seedlings and the expression of heat-inducible genes are enhanced by overexpression of GAPC and reduced in gapc. The effects of GAPC overexpression are abolished when NF-YC10 is deficient or GAPC nuclear accumulation is suppressed. Genetic complementation fails to recover the heat responses of gapc when GAPC-NF-YC10 interaction is disrupted. GAPC overexpression also enhances the binding ability of NF-YC10 to its target promoter. The results reveal a cellular and molecular mechanism for the nuclear moonlighting of a glycolytic enzyme in plant response to environmental changes.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_04:04:42.045.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD018945
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sangchul Kim
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	Oxidation; Acetyl; Carbamidomethyl; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-01 01:27:25	ID requested
⏵ 1	2021-09-09 04:04:42	announced

Kim SC, Guo L, Wang X, Nuclear moonlighting of cytosolic glyceraldehyde-3-phosphate dehydrogenase regulates Arabidopsis response to heat stress. Nat Commun, 11(1):3439(2020) [pubmed]

submitter keyword: GAPC, Arabidopsis, heat stress

Xuemin Wang
contact affiliation	University of Missouri-St. Louis
contact email	swang@danforthcenter.org
lab head
Sangchul Kim
contact affiliation	University of Missouri-St. Louis
contact email	skim@danforthcenter.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018945
     1. Label: PRIDE project
     2. Name: Nuclear moonlighting of cytosolic glyceraldehyde-3-phosphate dehydrogenase regulates Arabidopsis response to heat stress