PXD018942

PXD018942 is an original dataset announced via ProteomeXchange.

Title	Syndecan-2 partners in rat left ventricular lysates
Description	To get more insight into the role of syndecan-2, we here sought to identify interaction partners of syndecan-2 in rat left ventricle. By using three different affinity purification methods combined with mass spectrometry (AP-MS) analysis, we identified 30 novel partners and 9 partners previously described in the literature, which together make up the first cardiac syndecan-2 interactome. The three AP-MS were: 1) Immunoprecipitation of endogenous SDC2. Non-relevant IgG was used as negative control. 2) Fishing with recombinant GST-SDC2 (full length). GST and beads only were used as negative controls. 3) Pull down using a synthetic biotin-axh-SDC2cyt peptide (cytoplasmic tail). A scrambled peptide sequence and beads only were used as negative controls.
HostingRepository	PRIDE
AnnounceDate	2020-12-01
AnnouncementXML	Submission_2020-12-01_03:12:23.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tuula Nyman
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-30 16:46:41	ID requested
⏵ 1	2020-12-01 03:12:24	announced

Mathiesen SB, Lunde M, Stensland M, Martinsen M, Nyman TA, Christensen G, Carlson CR, The Cardiac Syndecan-2 Interactome. Front Cell Dev Biol, 8():792(2020) [pubmed]

submitter keyword: Syndecan-2, interactome, mass spectrometry

Tuula Nyman
contact affiliation	Head of Proteomics
contact email	tuula.nyman@medisin.uio.no
lab head
Tuula Nyman
contact affiliation	University of Oslo
contact email	tuula.nyman@medisin.uio.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD018942

PRIDE project URI

• PRIDE
  1. PXD018942
     1. Label: PRIDE project
     2. Name: Syndecan-2 partners in rat left ventricular lysates