PXD018941

PXD018941 is an original dataset announced via ProteomeXchange.

Title	Specific thylakoid protein phosphorylations are prerequisites for overwintering of Norway spruce (Picea abies) photosynthesis
Description	Coping of evergreen conifers of boreal forests with freezing temperatures on bright winter days puts the photosynthetic machinery in great risk of oxidative damage. To survive harsh winter conditions, conifers have evolved a unique but poorly characterised photoprotection mechanism, a sustained form of non-photochemical quenching (sustained NPQ). Here we focused on functional properties and underlying molecular mechanisms related to the development of sustained NPQ in Norway spruce (Picea abies). Data was collected during four consecutive years (2016-19) from trees growing in sun and shade habitats. When day temperatures dropped below -4°C, specific N-terminally triply phosphorylated LHCB1 isoform (3p-LHCII) and phosphorylated PSBS (p-PSBS) were detected in the thylakoid membrane. Development of sustained NPQ coincided with the highest level of 3p-LHCII and p-PSBS, occurring after prolonged combination of bright winter days and temperature close to -10°C. Artificial induction of both the sustained NPQ and recovery from naturally induced sustained NPQ provided information on differential dynamics and light-dependence of 3p-LHCII and p-PSBS accumulation and dephosphorylation as essential prerequisites of sustained NPQ. Data obtained collectively suggest three novel components related to sustained NPQ in spruce. (i) Freezing temperatures induce 3p-LHCII accumulation independently of light, which is suggested to initiate de-stacking of appressed thylakoid membranes due to increased electrostatic repulsion of adjacent membranes. (ii) p-PSBS accumulation is both light- and temperature-dependent and closely linked to the initiation of sustained NPQ, which (iii) in concert with PSII photoinhibition is likely to trigger sustained NPQ in spruce.
HostingRepository	PRIDE
AnnounceDate	2020-07-27
AnnouncementXML	Submission_2020-07-27_02:25:52.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD018941
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrea Trotta
SpeciesList	scientific name: Picea abies; NCBI TaxID: 3329;
ModificationList	Phospho; phosphorylated residue; Oxidation; Acetyl; acetylated residue; Carbamidomethyl; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-30 16:45:38	ID requested
⏵ 1	2020-07-27 02:25:53	announced

Grebe S, Trotta A, Bajwa AA, Mancini I, Bag P, Jansson S, Tikkanen M, Aro EM, ) photosynthesis. Proc Natl Acad Sci U S A, 117(30):17499-17509(2020) [pubmed]

submitter keyword: Picea abies, overwintering, cold acclimation, photosynthesis, thylakoids, phosphorylation, PSBS, LHCII, sustained NPQ

Eva-Mari Aro
contact affiliation	Molecular Plant Biology, Department of Biochemistry, University of Turku, Finland
contact email	evaaro@utu.fi
lab head
Andrea Trotta
contact affiliation	Molecular Plant Biology, Department of Biochemistry, University of Turku
contact email	andrea.trotta@utu.fi
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018941
     1. Label: PRIDE project
     2. Name: Specific thylakoid protein phosphorylations are prerequisites for overwintering of Norway spruce (Picea abies) photosynthesis