PXD018936

PXD018936 is an original dataset announced via ProteomeXchange.

Title	Proteomics of Naja naja venom from the Western Ghats of India
Description	This project mainly aims to characterize the complex toxic components present in the venom of Indian cobra (Naja naja) from the Western Ghats of India. Naja naja (NN) is native to the Indian subcontinent and is also found in Pakistan, Sri Lanka, Bangladesh and Southern Nepal. It is a highly venomous snake species of genus Naja of the Elapidae family. They are seen in wide habitats like plains, dense or open forests, rocky terrains, wetlands, agricultural lands, and outskirts of villages and even in highly populated urban areas. This species has been included in the ‘Big 4’ category of venomous snake species that accounts for majority of morbidity and mortality cases in India. Therefore, exploring the venom proteome of Naja naja is decisive to develop and design new antivenom and therapeutics against its envenomation. The venom proteome of Naja naja was characterized through various orthogonal separation strategies and identification strategies. In order to achieve this the crude venom components were resolved on a 12% SDS page. Also, the venom was decomplexed through reversed-phase HPLC followed by SDS analysis. Further each of the bands were subjected to in-gel digestion using trypsin, chymotrypsin and V8 proteases. All the digested peptides were then subjected to Q-TOF LC-MS/MS analysis.
HostingRepository	PRIDE
AnnounceDate	2024-06-20
AnnouncementXML	Submission_2024-06-20_00:57:23.098.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sudarslal Sadasivan Nair
SpeciesList	scientific name: Serpentes; NCBI TaxID: 8570;
ModificationList	No PTMs are included in the dataset
Instrument	6540 Q-TOF LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-30 06:26:11	ID requested
⏵ 1	2024-06-20 00:57:23	announced

10.1016/J.ACTATROPICA.2022.106324;

ProteomeXchange project tag: Toxicoproteomics (B/D-HPP)

submitter keyword: Venom proteomics, Western Ghats, Naja naja

Sudarslal Sadasivan Nair
contact affiliation	Associate Professor and Head-Proteomics Research, School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri Campus, Clappana P.O, Kollam - 690525 Kerala, India, Phone-0476-2803119, 2803110 (lab head)
contact email	sudarslal@gmail.com
lab head
Sudarslal Sadasivan Nair
contact affiliation	Amrita Vishwa VIdyapeetham
contact email	sudarslal@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD018936

PRIDE project URI

• PRIDE
  1. PXD018936
     1. Label: PRIDE project
     2. Name: Proteomics of Naja naja venom from the Western Ghats of India