PXD018934

PXD018934 is an original dataset announced via ProteomeXchange.

Title	New mode of methanogenesis observed in the coal degrading methanogen Methermicoccus shengliensis
Description	Methane-generating Archaea drive the final step in anaerobic organic compound mineralization and dictate the carbon flow of Earth’s diverse anaerobic ecosystems. Although such Archaea were presumed to be restricted to life on simple compounds like H2, acetate or methanol, an archaeon, Methermicoccus shengliensis, was recently found to convert methoxylated aromatic compounds to methane. Methoxylated aromatic compounds as component of lignin and coal are present in most subsurface sediments. Despite the significance and novelty of this outstanding archaeon its metabolism has not yet been explored. In this study, transcriptomics and proteomics reveal that M. shengliensis uses a demethoxylation system that is more related to that from acetogenic bacteria than to the methyl transferase system used for methylotrophic methanogenesis. It activates methoxy-groups using tetrahydromethanopterin as the carrier, a mechanism distinct from conventional methanogenic methyl-transfer systems dependent on Coenzyme M.
HostingRepository	PRIDE
AnnounceDate	2021-06-21
AnnouncementXML	Submission_2021-06-20_22:36:14.867.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julia Kurth
SpeciesList	scientific name: Methermicoccus shengliensis DSM 18856; NCBI TaxID: 1122233;
ModificationList	iodoacetamide derivatized amino-terminal residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-30 05:29:34	ID requested
1	2021-06-20 05:53:02	announced
⏵ 2	2021-06-20 22:36:15	announced	2021-06-21: Updated project metadata.

Kurth JM, Nobu MK, Tamaki H, de Jonge N, Berger S, Jetten MSM, Yamamoto K, Mayumi D, Sakata S, Bai L, Cheng L, Nielsen JL, Kamagata Y, Wagner T, Welte CU, Methanogenic archaea use a bacteria-like methyltransferase system to demethoxylate aromatic compounds. ISME J, 15(12):3549-3565(2021) [pubmed]

10.1038/S41396-021-01025-6;

submitter keyword: demethoxylation, methyl transfer,Methanogen, methoxylated aromatic compounds

Jeppe Lund Nielsen
contact affiliation	Department of Chemistry and Bioscience, Aalborg University, Denmark
contact email	jln@bio.aau.dk
lab head
Julia Kurth
contact affiliation	Radboud University
contact email	j.kurth@science.ru.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD018934

PRIDE project URI

• PRIDE
  1. PXD018934
     1. Label: PRIDE project
     2. Name: New mode of methanogenesis observed in the coal degrading methanogen Methermicoccus shengliensis