PXD018921

PXD018921 is an original dataset announced via ProteomeXchange.

Title	HDX-MS analysis of the full human proteasome in complex with regulators
Description	Hydrogen-Deuterium eXchange coupled to Mass Spectrometry (HDX-MS) analysis to investigate the solvent accessibility of the full human 20S proteasome alone or in complex with PA28 regulatory particles. Our work reveals a reciprocal crosstalk between the inner and outer rings of the human 20S proteasome. HDX-MS experiments were performed on a Synapt-G2Si (Waters Scientific, Manchester, UK) coupled to a Twin HTS PAL dispensing and labelling robot (LEAP Technologies, Carborro, NC, USA) via a NanoAcquity system with HDX technology (Waters, Manchester, UK).
HostingRepository	PRIDE
AnnounceDate	2020-05-07
AnnouncementXML	Submission_2020-05-07_02:32:02.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julien Marcoux
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-29 22:32:01	ID requested
⏵ 1	2020-05-07 02:33:17	announced

Dataset with its publication pending

submitter keyword: : proteasome, immunoproteasome, PA28 alpha beta, PA28 gamma, hydrogen-deuterium exchange, structural MS, bioinformatics, structural biology

Julien Marcoux
contact affiliation	Proteomics and Mass Spectrometry of Biomolecules, Institut de Pharmacologie et de Biologie Structurale (IPBS), Toulouse ,France
contact email	julien.marcoux@ipbs.fr
lab head
Julien Marcoux
contact affiliation	IPBS
contact email	julien.marcoux@ipbs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD018921

PRIDE project URI

• PRIDE
  1. PXD018921
     1. Label: PRIDE project
     2. Name: HDX-MS analysis of the full human proteasome in complex with regulators