PXD018918 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A PEROXO-Tag enables rapid isolation of peroxisomes from human cells |
Description | Peroxisomes are metabolic organelles that perform a diverse array of critical functions in human physiology. Traditional isolation methods for peroxisomes can take more than one hour to complete and can be laborious to implement. To address this, we have now extended our prior work on rapid organellar isolation to peroxisomes via the development of a peroxisomally-localized 3XHA epitope tag (“PEROXO-Tag”) and associated immunoprecipitation (“PEROXO-IP”) workflow. Our PEROXO-IP workflow has excellent reproducibility, is easy to implement, and achieves highly rapid (~10 minutes post-homogenization) and specific isolation of human peroxisomes, which we characterize here via proteomic profiling. By offering speed, specificity, reproducibility, and ease of use, the PEROXO-IP workflow should facilitate studies on the biology of peroxisomes. |
HostingRepository | PRIDE |
AnnounceDate | 2020-05-20 |
AnnouncementXML | Submission_2020-05-19_22:56:02.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD018918 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Elizabeth Boydston |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Phospho; methylthiolated residue; phosphorylated residue; Oxidation; Acetyl; acetylated residue; monohydroxylated residue; Carbamidomethyl |
Instrument | Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-04-29 17:47:50 | ID requested | |
⏵ 1 | 2020-05-19 22:56:03 | announced | |
Publication List
Ray GJ, Boydston EA, Shortt E, Wyant GA, Lourido S, Chen WW, Sabatini DM, A PEROXO-Tag Enables Rapid Isolation of Peroxisomes from Human Cells. iScience, 23(5):101109(2020) [pubmed] |
Keyword List
submitter keyword: Human HEK-293T, peroxisome |
Contact List
Sebastian Lourido |
contact affiliation | Whitehead Institute for Biomedical Research, Cambridge, MA; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA |
contact email | lourido@wi.mit.edu |
lab head | |
Elizabeth Boydston |
contact affiliation | Whitehead Institute for Biomedical Research |
contact email | liz@wi.mit.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD018918 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018918
- Label: PRIDE project
- Name: A PEROXO-Tag enables rapid isolation of peroxisomes from human cells