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PXD018909

PXD018909 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics of Galápagos marine iguanas links function of femoral gland proteins to the immune system
DescriptionCommunication between individuals via molecules, termed chemosignaling, is widespread among animal and plant species. However, we lack knowledge on the specific functions of the substances involved for most of such systems. The femoral gland is an organ secreting a waxy substance, which is involved in chemical communication in lizards. While the lipids and volatile substances secreted by the femoral glands have been investigated in several biochemical studies, the protein composition and functions of secretions are completely unknown. Applying a proteomic approach, we provide the first attempt to comprehensively characterize the protein composition of femoral gland secretions from the Galápagos marine iguana. Using samples from several organs, the marine iguana proteome was assembled by next generation sequencing and mass spectrometry, resulting in 7,513 proteins; 4305 proteins were present in the femoral gland, including keratins, small serum proteins and fatty acid-binding proteins. Surprisingly, no proteins with obvious roles in partner recognition or inter-species communication could be identified. However, we found several proteins with direct associations to the innate immune system, such as lysozyme C, antileukoproteinase (ALP), pulmonary surfactant protein (SFTPD) and galectin (LGALS1), suggesting the femoral glands function as an important barrier to infection. The identification of previously unrecognized effective anti-microbial peptides from the femoral gland show similar activities against E.coli and B. subtilis compared to oncocin. This proteomics dataset is a valuable resource for future functional protein analysis and demonstrates that femoral glands secretions covers also functions of the innate immune system.
HostingRepositoryPRIDE
AnnounceDate2020-06-29
AnnouncementXMLSubmission_2020-06-29_03:49:14.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFrederik Tellkamp
SpeciesList scientific name: Amblyrhynchus cristatus; NCBI TaxID: 51208;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-04-29 15:03:36ID requested
12020-06-29 03:49:15announced
Publication List
Tellkamp F, Lang F, Ibáñez Ricoma A, Abraham L, Quezada G, Günther S, Looso M, Tann FJ, Müller D, Cemic F, Hemberger J, Steinfartz S, Krüger M, Proteomics of Galápagos marine iguanas links function of femoral gland proteins to the immune system. Mol Cell Proteomics, ():(2020) [pubmed]
Keyword List
submitter keyword: Iguana Proteomics Galapagos femoral gland immune
Contact List
Marcus Krüger
contact affiliationCECAD Research Center, Institute for Genetics, University of Cologne, Cologne, Germany
contact emailmarcus.krueger@uni-koeln.de
lab head
Frederik Tellkamp
contact affiliationUniversity of Cologne
contact emailftellka1@uni-koeln.de
dataset submitter
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