PXD018909

PXD018909 is an original dataset announced via ProteomeXchange.

Title	Proteomics of Galápagos marine iguanas links function of femoral gland proteins to the immune system
Description	Communication between individuals via molecules, termed chemosignaling, is widespread among animal and plant species. However, we lack knowledge on the specific functions of the substances involved for most of such systems. The femoral gland is an organ secreting a waxy substance, which is involved in chemical communication in lizards. While the lipids and volatile substances secreted by the femoral glands have been investigated in several biochemical studies, the protein composition and functions of secretions are completely unknown. Applying a proteomic approach, we provide the first attempt to comprehensively characterize the protein composition of femoral gland secretions from the Galápagos marine iguana. Using samples from several organs, the marine iguana proteome was assembled by next generation sequencing and mass spectrometry, resulting in 7,513 proteins; 4305 proteins were present in the femoral gland, including keratins, small serum proteins and fatty acid-binding proteins. Surprisingly, no proteins with obvious roles in partner recognition or inter-species communication could be identified. However, we found several proteins with direct associations to the innate immune system, such as lysozyme C, antileukoproteinase (ALP), pulmonary surfactant protein (SFTPD) and galectin (LGALS1), suggesting the femoral glands function as an important barrier to infection. The identification of previously unrecognized effective anti-microbial peptides from the femoral gland show similar activities against E.coli and B. subtilis compared to oncocin. This proteomics dataset is a valuable resource for future functional protein analysis and demonstrates that femoral glands secretions covers also functions of the innate immune system.
HostingRepository	PRIDE
AnnounceDate	2020-06-29
AnnouncementXML	Submission_2020-06-29_03:49:14.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frederik Tellkamp
SpeciesList	scientific name: Amblyrhynchus cristatus; NCBI TaxID: 51208;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-29 15:03:36	ID requested
⏵ 1	2020-06-29 03:49:15	announced

Tellkamp F, Lang F, Ib, á, ñ, ez A, Abraham L, Quezada G, G, ü, nther S, Looso M, Tann FJ, M, ü, ller D, Cemic F, Hemberger J, Steinfartz S, Kr, ü, ger M, pagos Marine Iguanas Links Function of Femoral Gland Proteins to the Immune System. Mol Cell Proteomics, 19(9):1523-1532(2020) [pubmed]

submitter keyword: Iguana Proteomics Galapagos femoral gland immune

Marcus Krüger
contact affiliation	CECAD Research Center, Institute for Genetics, University of Cologne, Cologne, Germany
contact email	marcus.krueger@uni-koeln.de
lab head
Frederik Tellkamp
contact affiliation	University of Cologne
contact email	ftellka1@uni-koeln.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018909
     1. Label: PRIDE project
     2. Name: Proteomics of Galápagos marine iguanas links function of femoral gland proteins to the immune system