PXD018895

PXD018895 is an original dataset announced via ProteomeXchange.

Title	Benchmarking of different methods for obtaining high quality secretomics data
Description	The main drawback of secretome analyses are contaminating proteins derived from culture medium or proteins released from dying cells which are hard to distinguish from proteins actively secreted by cells. Therefore, several approaches have been applied allowing to remove contamination from secretome analysis. Here, we will compare these approaches and will evaluate their potential to determine proteins secreted by unconventional secretory pathways.
HostingRepository	PRIDE
AnnounceDate	2020-10-19
AnnouncementXML	Submission_2020-10-19_00:39:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gereon Poschmann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-29 03:32:55	ID requested
⏵ 1	2020-10-19 00:39:55	announced

Poschmann G, Brenig K, Lenz T, St, ü, hler K, Comparative Secretomics Gives Access to High Confident Secretome Data: Evaluation of Different Methods for the Determination of Bona Fide Secreted Proteins. Proteomics, 21(2):e2000178(2021) [pubmed]

submitter keyword: secreteome, secretomics, conditioned medium, supernatant, benchmarking

Gereon Poschmann
contact affiliation	Proteome Research (AG Stühler) Heinrich Heine University Düsseldorf Universitätsstraße 1 40225 Düsseldorf
contact email	gereon.poschmann@hhu.de
lab head
Gereon Poschmann
contact affiliation	Molecular Proteomics Laboratory
contact email	gereon.poschmann@hhu.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD018895

PRIDE project URI

• PRIDE
  1. PXD018895
     1. Label: PRIDE project
     2. Name: Benchmarking of different methods for obtaining high quality secretomics data