PXD018881
PXD018881 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | ACONITASE 3 is part of the ANAC017 transcription factor-dependent mitochondrial dysfunction response |
Description | Mitochondria are tightly embedded within metabolic and regulatory networks that optimize plant performance in response to environmental challenges. The best-known mitochondrial retrograde signaling pathway involves stress-induced activation of the transcription factor NAC DOMAIN CONTAINING PROTEIN 17 (ANAC017), which initiates protective responses to stress-induced mitochondrial dysfunction in Arabidopsis (Arabidopsis thaliana). Post-translational control of the elicited responses, however, remains poorly understood. Previous studies linked protein phosphatase 2A subunit PP2A-B’γ, a key negative regulator of stress responses, with reversible phosphorylation of ACONITASE 3 (ACO3). Here we report on ACO3 and its phosphorylation at Ser91 as key components of stress regulation that are induced by mitochondrial dysfunction. Targeted mass spectrometry-based proteomics revealed that the abundance and phosphorylation of ACO3 increased under stress, which required signaling through ANAC017. Phosphomimetic mutation at ACO3-Ser91 and accumulation of ACO3S91D-YFP promoted the expression of genes related to mitochondrial dysfunction. Furthermore, ACO3 contributed to plant tolerance against UV-B or antimycin A-induced mitochondrial dysfunction. These findings demonstrate that ACO3 is both a target and mediator of mitochondrial dysfunction signaling, and critical for achieving stress tolerance in Arabidopsis leaves. |
HostingRepository | PanoramaPublic |
AnnounceDate | 2022-03-14 |
AnnouncementXML | Submission_2022-03-14_21:15:32.355.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Jesús Pascual |
SpeciesList | scientific name: Arabidopsis thaliana; NCBI TaxID: 3702; |
ModificationList | Phospho; Carbamidomethyl |
Instrument | Q Exactive; Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-04-28 19:59:35 | ID requested | |
1 | 2021-05-14 07:16:51 | announced | |
⏵ 2 | 2022-03-14 21:15:33 | announced | : Added PubMed Id |
Publication List
Pascual J, Rahikainen M, Angeleri M, Alegre S, Gossens R, Shapiguzov A, Heinonen A, Trotta A, Durian G, Winter Z, Sinkkonen J, Kangasj, ä, rvi J, Whelan J, Kangasj, ä, rvi S, ACONITASE 3 is part of theANAC017 transcription factor-dependent mitochondrial dysfunction response. Plant Physiol, 186(4):1859-1877(2021) [pubmed] |
Keyword List
submitter keyword: aconitase, mitochondrial dysfunction response, phosphorylation, post-translational regulation |
Contact List
Saijaliisa Kangasjärvi | |
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contact affiliation | Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki |
contact email | saijaliisa.kangasjärvi@helsinki.fi |
lab head | |
Jesús Pascual | |
contact affiliation | Molecular Plant Biology, Department of Biochemistry, University of Turku, FI-20014 Turku, Finland |
contact email | pascualjesus@uniovi.es |
dataset submitter |
Full Dataset Link List
Panorama Public dataset URI |