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PXD018881

PXD018881 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleACONITASE 3 is part of the ANAC017 transcription factor-dependent mitochondrial dysfunction response
DescriptionMitochondria are tightly embedded within metabolic and regulatory networks that optimize plant performance in response to environmental challenges. The best-known mitochondrial retrograde signaling pathway involves stress-induced activation of the transcription factor NAC DOMAIN CONTAINING PROTEIN 17 (ANAC017), which initiates protective responses to stress-induced mitochondrial dysfunction in Arabidopsis (Arabidopsis thaliana). Post-translational control of the elicited responses, however, remains poorly understood. Previous studies linked protein phosphatase 2A subunit PP2A-B’γ, a key negative regulator of stress responses, with reversible phosphorylation of ACONITASE 3 (ACO3). Here we report on ACO3 and its phosphorylation at Ser91 as key components of stress regulation that are induced by mitochondrial dysfunction. Targeted mass spectrometry-based proteomics revealed that the abundance and phosphorylation of ACO3 increased under stress, which required signaling through ANAC017. Phosphomimetic mutation at ACO3-Ser91 and accumulation of ACO3S91D-YFP promoted the expression of genes related to mitochondrial dysfunction. Furthermore, ACO3 contributed to plant tolerance against UV-B or antimycin A-induced mitochondrial dysfunction. These findings demonstrate that ACO3 is both a target and mediator of mitochondrial dysfunction signaling, and critical for achieving stress tolerance in Arabidopsis leaves.
HostingRepositoryPanoramaPublic
AnnounceDate2021-05-14
AnnouncementXMLSubmission_2021-05-14_07:16:51.329.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJesús Pascual
SpeciesList scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationListPhospho; Carbamidomethyl
InstrumentQ Exactive; Q Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-04-28 19:59:35ID requested
12021-05-14 07:16:51announced
Publication List
Pascual J, Rahikainen M, Angeleri M, Alegre S, Gossens R, Shapiguzov A, Heinonen A, Trotta A, Durian G, Winter Z, Sinkkonen J, Kangasjärvi J, Whelan J, Kangasjärvi S. ACONITASE 3 is part of the ANAC017 transcription factor-dependent mitochondrial dysfunction response. Plant Physiol [Internet]. 2021 May 12
Available from: https://doi.org/10.1093/plphys/kiab225
Keyword List
submitter keyword: aconitase, mitochondrial dysfunction response, phosphorylation, post-translational regulation
Contact List
Saijaliisa Kangasjärvi
contact affiliationOrganismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki
contact emailsaijaliisa.kangasjärvi@helsinki.fi
lab head
Jesús Pascual
contact affiliationMolecular Plant Biology, Department of Biochemistry, University of Turku, FI-20014 Turku, Finland
contact emailjepavaz@utu.fi
dataset submitter
Full Dataset Link List
Panorama Public dataset URI