PXD018880

PXD018880 is an original dataset announced via ProteomeXchange.

Title	A PEROXO-Tag enables rapid isolation of peroxisomes from human cells
Description	Peroxisomes are metabolic organelles that perform a diverse array of critical functions in human physiology. Traditional isolation methods for peroxisomes can take more than one hour to complete and can be laborious to implement. To address this, we have now extended our prior work on rapid organellar isolation to peroxisomes via the development of a peroxisomally-localized 3XHA epitope tag (“PEROXO-Tag”) and associated immunoprecipitation (“PEROXO-IP”) workflow. Our PEROXO-IP workflow has excellent reproducibility, is easy to implement, and achieves highly rapid (~10 minutes post-homogenization) and specific isolation of human peroxisomes, which we characterize here via proteomic profiling. By offering speed, specificity, reproducibility, and ease of use, the PEROXO-IP workflow should facilitate studies on the biology of peroxisomes.
HostingRepository	PRIDE
AnnounceDate	2020-05-20
AnnouncementXML	Submission_2020-05-19_23:28:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Elizabeth Boydston
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	methylthiolated residue; phosphorylated residue; acetylated residue; monohydroxylated residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-28 17:17:58	ID requested
⏵ 1	2020-05-19 23:28:46	announced

Ray GJ, Boydston EA, Shortt E, Wyant GA, Lourido S, Chen WW, Sabatini DM, A PEROXO-Tag Enables Rapid Isolation of Peroxisomes from Human Cells. iScience, 23(5):101109(2020) [pubmed]

submitter keyword: Human HEK-293T, peroxisome, PEROXO-IP

Sebastian Lourido
contact affiliation	Whitehead Institute for Biomedical Research, Cambridge, MA; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA
contact email	lourido@wi.mit.edu
lab head
Elizabeth Boydston
contact affiliation	Whitehead Institute for Biomedical Research
contact email	liz@wi.mit.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD018880

PRIDE project URI

• PRIDE
  1. PXD018880
     1. Label: PRIDE project
     2. Name: A PEROXO-Tag enables rapid isolation of peroxisomes from human cells