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PXD018872

PXD018872 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEvaluation of proteomics strategies for low cell numbers: SP3- and C18-based protocols combined with five cell lysis methods
DescriptionNew advances in the transcriptomics and flow cytometry fields have allowed the discovery of new immune cell subsets. However, most of them are extremely small populations, hampering their analysis using mass spectrometry strategies since currently these procedures are not highly efficient in small sample sizes. In this context, recent studies have improved sample processing for quantity-limited samples. Here, we aim at evaluating the combination of five cell lysis methods and two sample clean-up techniques (i.e. C18 and SP3) for the analysis of small cell numbers (2.500, 10.000, 50.000) of THP-1, differentiated THP-1 and HT-29 cell lines.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:51:02.034.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD018872
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterPaula Díez
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListAmmonia-loss; Glu->pyro-Glu; Oxidation; Acetyl; Carbamidomethyl; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; Gln->pyro-Glu
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-04-28 16:30:12ID requested
12023-02-09 11:42:57announced
22023-11-14 08:51:02announced2023-11-14: Updated project metadata.
Publication List
10.6019/PXD018872;
Keyword List
submitter keyword: Human, HT-29, C18, macrophages, low cell number, SP3, THP-1, small populations
Contact List
Paula Díez, PhD
contact affiliationImmune monitoring lab Department of Immunohematology and Blood transfusion Leiden University Medical Center (LUMC) The Netherlands
contact emailp.diez_garcia@lumc.nl
lab head
Paula Díez
contact affiliationImmune monitoring group - Immunohematology and Blood Transfusion Department Leiden University Medical Center (LUMC=
contact emailp.diez_garcia@lumc.nl
dataset submitter
Full Dataset Link List
Dataset FTP location
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