PXD018872 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Evaluation of proteomics strategies for low cell numbers: SP3- and C18-based protocols combined with five cell lysis methods |
Description | New advances in the transcriptomics and flow cytometry fields have allowed the discovery of new immune cell subsets. However, most of them are extremely small populations, hampering their analysis using mass spectrometry strategies since currently these procedures are not highly efficient in small sample sizes. In this context, recent studies have improved sample processing for quantity-limited samples. Here, we aim at evaluating the combination of five cell lysis methods and two sample clean-up techniques (i.e. C18 and SP3) for the analysis of small cell numbers (2.500, 10.000, 50.000) of THP-1, differentiated THP-1 and HT-29 cell lines. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:51:02.034.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD018872 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Paula Díez |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Ammonia-loss; Glu->pyro-Glu; Oxidation; Acetyl; Carbamidomethyl; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; Gln->pyro-Glu |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-04-28 16:30:12 | ID requested | |
1 | 2023-02-09 11:42:57 | announced | |
⏵ 2 | 2023-11-14 08:51:02 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: Human, HT-29, C18, macrophages, low cell number, SP3, THP-1, small populations |
Contact List
Paula Díez, PhD |
contact affiliation | Immune monitoring lab Department of Immunohematology and Blood transfusion Leiden University Medical Center (LUMC) The Netherlands |
contact email | p.diez_garcia@lumc.nl |
lab head | |
Paula Díez |
contact affiliation | Immune monitoring group - Immunohematology and Blood Transfusion Department Leiden University Medical Center (LUMC= |
contact email | p.diez_garcia@lumc.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD018872 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018872
- Label: PRIDE project
- Name: Evaluation of proteomics strategies for low cell numbers: SP3- and C18-based protocols combined with five cell lysis methods