PXD018867

PXD018867 is an original dataset announced via ProteomeXchange.

Title	Insights into the natural defences of a coral reef fish against gill ectoparasites: integrated metabolome and microbiome approach.
Description	Understanding natural defence mechanisms against parasites can be a valuable tool for the development of innovative therapies. In this study, we investigated the interplay between the gill mucus metabolome and microbiome of Chaetodon lunulatus, a butterflyfish known to avoid gill monogeneans whilst living amongst closely related parasitized species. In an attempt to identify metabolites and OTUs potentially involved in parasite defence mechanisms, we studied the metabolome (LC-MS/MS) and microbiome of several sympatric butterflyfish species, including the only non-parasitized species C. lunulatus. After observing significant differences between the metabolome and microbiome of parasitized versus non-parasitized fish (PCoA, ANOSIM), we obtained the discriminant metabolites and OTUs using a supervised analysis. Some of the most important discriminant metabolites were identified as peptides, and three new β-subunit haemoblogin-derived peptides from C. lunulatus (CLHbβ-1, CLHbβ-2 and CLHbβ-3) were purified, characterised and synthesised. We also identified specific bacterial families and OTUs typical from low-oxygen habitats in C. lunulatus gill mucus. By using a correlation network between the two datasets, we found a Fusobacteriaceae strain exclusively present in C. lunulatus highly correlated to the peptides. Finally, we discuss the possible involvement of these peptides and Fusobacteriaceae in monogenean avoidance by this fish species.
HostingRepository	PRIDE
AnnounceDate	2020-11-25
AnnouncementXML	Submission_2020-11-25_07:01:21.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD018867
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sebastien Voisin
SpeciesList	scientific name: Chaetodon lunulatus; NCBI TaxID: 281677;
ModificationList	Met-loss; Oxidation
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-28 10:26:16	ID requested
⏵ 1	2020-11-25 07:01:22	announced

Reverter M, Sasal P, Suzuki MT, Raviglione D, Inguimbert N, Pare A, Banaigs B, Voisin SN, Bulet P, Tapissier-Bontemps N, Insights into the Natural Defenses of a Coral Reef Fish Against Gill Ectoparasites: Integrated Metabolome and Microbiome Approach. Metabolites, 10(6):(2020) [pubmed]

submitter keyword: fish mucus, host-parasite interactions, butterflyfish, metabolomics, microbiome, multi-omics, Chaetodon

Philippe Bulet
contact affiliation	CR Université Grenoble Alpes, Institute for Advanced Biosciences, INSERM U1209, CNRS UMR5309, La Tronche, France.
contact email	philippe.bulet@univ-grenoble-alpes.fr
lab head
Sebastien Voisin
contact affiliation	Plateforme BioPark of Archamps
contact email	sebastien.voisin@biopark-archamps.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD018867

PRIDE project URI

• PRIDE
  1. PXD018867
     1. Label: PRIDE project
     2. Name: Insights into the natural defences of a coral reef fish against gill ectoparasites: integrated metabolome and microbiome approach.