PXD018833

PXD018833 is an original dataset announced via ProteomeXchange.

Title	Overexpression of sedoheptulose-1,7-bisphosphatase enhances photosynthesis in Chlamydomonas reinhardtii and has no effect on the abundance of other Calvin-Benson cycle enzymes
Description	The productivity of plants and microalgae needs to be increased to feed the growing world population and to promote the development of a low-carbon economy. This goal can be achieved by improving photosynthesis via genetic engineering. In this study, we have employed the Modular Cloning strategy to overexpress the Calvin-Benson cycle (CBC) enzyme sedoheptulose-1,7 bisphosphatase (SBP1) up to 3-fold in the unicellular green alga Chlamydomonas reinhardtii. The protein derived from the nuclear transgene represented ~0.3% of total cell protein. Photosynthetic rate and growth were significantly increased in SBP1-overexpressing lines under high-light and high-CO2 conditions. Absolute quantification of the abundance of all other CBC enzymes by the QconCAT approach revealed no consistent differences between SBP1-overexpressing lines and the recipient strain. This analysis also revealed that the eleven CBC enzymes represent 11.9% of total cell protein in Chlamydomonas. Here the range of concentrations of CBC enzymes turned out to be much larger than estimated earlier, with a 128-fold difference between the most abundant CBC protein (rbcL) and the least abundant (triose phosphate isomerase). Accordingly, the concentrations of the CBC intermediates are often but not always higher than the binding site concentrations of the enzymes for which they act as substrates. The enzymes with highest substrate to binding site ratios might represent good candidates for overexpression in subsequent engineering steps.
HostingRepository	PRIDE
AnnounceDate	2021-02-12
AnnouncementXML	Submission_2021-02-11_22:31:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Zimmer
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	monohydroxylated residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-27 08:17:20	ID requested
1	2021-02-11 08:08:27	announced
⏵ 2	2021-02-11 22:31:41	announced	2021-02-12: Updated project metadata.

10.3389/FPLS.2020.00868;

Hammel A, Sommer F, Zimmer D, Stitt M, M, ü, hlhaus T, Schroda M, and Has No Effect on the Abundance of Other Calvin-Benson Cycle Enzymes. Front Plant Sci, 11():868(2020) [pubmed]

submitter keyword: Mass Spectrometry, proteotypic peptide, QconCAT, Synthetic Biology, Modular cloning, Photosynthesis, Chlamydomonas reinhardtii

Michael Schroda
contact affiliation	Molekulare Biotechnologie und Systembiologie, Technische Universität Kaiserslautern, Kaiserslautern, Germany
contact email	schroda@bio.uni-kl.de
lab head
David Zimmer
contact affiliation	TU KL - CSB
contact email	davidzimmer91@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD018833

PRIDE project URI

• PRIDE
  1. PXD018833
     1. Label: PRIDE project
     2. Name: Overexpression of sedoheptulose-1,7-bisphosphatase enhances photosynthesis in Chlamydomonas reinhardtii and has no effect on the abundance of other Calvin-Benson cycle enzymes