PXD018832

PXD018832 is an original dataset announced via ProteomeXchange.

Title	Primary myeloid cell proteomics and transcriptomics: importance of β-tubulin isotypes for osteoclast function
Description	Among hematopoietic cells, osteoclasts (Oc) and immature dendritic cells (Dc) are closely related myeloid cells with distinct functions; Oc participate skeleton maintenance while Dc sample the environment for foreign antigens. Such specificities rely on profound modifications of gene and protein expression during Oc and Dc differentiation. We provide global proteomic and transcriptomic analyses of primary mouse Oc and Dc, based on original SILAC and RNAseq data. We established specific signatures for Oc and Dc including genes and proteins of unknown functions. In particular, we showed that Oc and Dc have the same alpha and beta tubulin isotypes repertoire but that Oc express much more beta tubulin isotype Tubb6 (also known as Tubulin beta class V). In both mouse and human Oc, we demonstrate that elevated expression of Tubb6 in Oc is necessary for correct podosomes organization and thus for the structure of the sealing zone, which sustains the bone resorption apparatus. Hence, lowering Tubb6 expression hindered Oc resorption activity. Overall, we highlight here potential new regulators of Oc and Dc biology and illustrate the functional importance of the tubulin isotype repertoire in the biology of differentiated cells.
HostingRepository	PRIDE
AnnounceDate	2020-09-16
AnnouncementXML	Submission_2020-09-16_06:29:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oana Vigy
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-27 08:17:20	ID requested
⏵ 1	2020-09-16 06:29:51	announced

Gu, é, rit D, Marie P, Morel A, Maurin J, Verollet C, Raynaud-Messina B, Urbach S, Blangy A, -tubulin isotypes for osteoclast function. J Cell Sci, 133(10):(2020) [pubmed]

submitter keyword: Osteoclast, Tubulin, SILAC, RNAseq, Myeloid cell, Tubb6, Tubulin beta class V

Anne Blangy
contact affiliation	Centre de Recherche en Biologie cellulaire de Montpellier (CRBM), CNRS UMR 5237, Montpellier University, France
contact email	anne.blangy@crbm.cnrs.fr
lab head
Oana Vigy
contact affiliation	FPP CNRS
contact email	oana.vigy@fpp.cnrs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD018832

PRIDE project URI

• PRIDE
  1. PXD018832
     1. Label: PRIDE project
     2. Name: Primary myeloid cell proteomics and transcriptomics: importance of β-tubulin isotypes for osteoclast function