PXD018823

PXD018823 is an original dataset announced via ProteomeXchange.

Title	BLM complex composition in unperturbed cells
Description	The Bloom syndrome DNA helicase BLM contributes to chromosome stability through its roles in double-strand break repair by homologous recombination and DNA replication fork restart during the replication stress response. Loss of BLM activity leads to Bloom syndrome, which is characterized by extraordinary cancer risk and small stature. Here, we have analyzed the composition of the BLM complex in unperturbed cells and identified a direct physical interaction with the Mcm6 subunit of the minichromosome maintenance (MCM) complex by co-immunopecipitations using endogenous and recombinant proteins as well as two-hybrid analysis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:31:49.643.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kristina Schmidt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-27 01:01:50	ID requested
1	2022-02-15 11:02:26	announced
⏵ 2	2024-10-22 05:31:56	announced	2024-10-22: Updated project metadata.

Shastri VM, Subramanian V, Schmidt KH, A novel cell-cycle-regulated interaction of the Bloom syndrome helicase BLM with Mcm6 controls replication-linked processes. Nucleic Acids Res, 49(15):8699-8713(2021) [pubmed]

10.1093/nar/gkab663;

submitter keyword: GM00637, Q Exactive Plus,human

Kristina Hildegard Schmidt
contact affiliation	Department of Cell Biology, Microbiology and Molecular Biology University of South Florida Tampa, Florida
contact email	kschmidt@usf.edu
lab head
Kristina Schmidt
contact affiliation	University of South Florida
contact email	kschmidt@usf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD018823

PRIDE project URI

• PRIDE
  1. PXD018823
     1. Label: PRIDE project
     2. Name: BLM complex composition in unperturbed cells