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PXD018819

PXD018819 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHerpes simplex virus infections of cortical brain cells: the proteome and secretome of the host
DescriptionHerpes simplex virus-1 (HSV-1) infections of the brain cause a severe neuroinflammatory condition – Herpes simplex encephalitis (HSE). During the acute phase, HSV-1 changes the host cells to facilitate its own replication and to escape host immunity. However, the host reacts with a severe neuroinflammation which may be beneficial for viral clearance or detrimental inducing neuronal damage and hindering regeneration. Indeed, surviving HSE-patients often suffer from an incomplete regeneration with memory deficits and other neuropsychiatric symptoms. HSV-1 infects different cell types such as neurons, astrocytes, and oligodendrocytes all of which participate in the host response. This includes a changed paracrine signaling. However, the host response has not been characterized on a global molecular level so far. Here, we infected primary mixed cortical cells with HSV-1 and analyzed the proteome and the secretome of the host. We identified 28 differentially regulated proteins in the host proteome involved in endocytosis, vesicle trafficking at the golgi-apparatus, and inflammasome formation. In the secretome, we identified 71 differentially regulated proteins with a subset involved in axonal regeneration. Endocytosis and vesicle trafficking are critical for viral entry and envelopment. The suppression of inflammasome activity is crucial for viral spread. Thus, our proteomic analysis hints for potential molecules regulating viral production and spread in brain cells. Moreover, the secretome analysis gives rise to a set of proteins involved in neuroregeneration.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:27:25.361.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAndreas Picjh
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListS-carboxamidoethyl-L-cysteine; monohydroxylated residue; acetylated residue; deamidated residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-04-27 01:01:25ID requested
12021-09-08 18:46:39announced
22024-10-22 05:27:26announced2024-10-22: Updated project metadata.
Publication List
10.3389/fneur.2020.00844;
Hensel N, Raker V, F, ö, rthmann B, Buch A, Sodeik B, Pich A, Claus P, The Proteome and Secretome of Cortical Brain Cells Infected With Herpes Simplex Virus. Front Neurol, 11():844(2020) [pubmed]
Keyword List
submitter keyword: ArfGap1, glutathione, encephalitis, Nek7, inflammasome, HSV-1, semaphorin,Herpes simplex virus-1, Rab GTPases
Contact List
Andreas Pich
contact affiliationHannover Medical School
contact emailpich.andreas@mh-hannover.de
lab head
Andreas Picjh
contact affiliationHannover Medical School Core Facility Proteomics
contact emailpich.andreas@mh-hannover.de
dataset submitter
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