PXD018817
PXD018817 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Neuronal cell selective proteomics in C. elegans |
Description | Neurons are categorised into many subclasses, and each subclass displays different morphology, expression patterns, connectivity and function. Changes in protein synthesis are critical for neuronal function. Therefore, analysing protein expression patterns in individual neuronal subclass will elucidate molecular mechanisms for memory and other functions. In this study, we used neuronal subclass-selective proteomic analysis with cell-selective Bio-Orthogonal Non-Canonical Amino acid Tagging (BONCAT). We selected Caenorhabditis elegans as a model organism because it shows diverse neuronal functions and simple neural circuitry. We performed proteomic analysis of all neurons or AFD subclass neurons that regulate thermotaxis in C. elegans. Mutant phenylalanyl tRNA synthetase (MuPheRS) was selectively expressed in all neurons or AFD subclass neurons, and azido-phenylalanine was incorporated into proteins in cells of interest. Azide-labelled proteins were enriched and proteomic analysis was performed. We identified 4412 and 1834 proteins from strains producing MuPheRS in all neurons and AFD subclass neuron, respectively. F23B2.10 (RING-type domain-containing protein) was identified only in neuronal cell-enriched proteomic analysis. We expressed GFP under the control of the 5' regulatory region of F23B2.10 and found GFP expression in neurons. We expect that more single-neuron specific proteomic data will clarify how protein composition and abundance affect characteristics of neuronal subclasses. |
HostingRepository | jPOST |
AnnounceDate | 2020-08-10 |
AnnouncementXML | Submission_2022-09-18_02:48:55.419.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Aburaya Shunsuke |
SpeciesList | scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; |
ModificationList | S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-04-27 00:13:57 | ID requested | |
1 | 2020-08-09 08:00:05 | announced | |
2 | 2021-02-04 17:23:10 | announced | 2021-02-04: Updated FTP location. |
⏵ 3 | 2022-09-18 02:48:55 | announced | 2022-09-18: Updated FTP location. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: BONCAT, C. elegans, Neuron |
Contact List
Wataru Aoki | |
---|---|
lab head | |
Aburaya Shunsuke | |
contact affiliation | Kyushu University |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000836/ |