PXD018810
PXD018810 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Thermal proteome profiling of Toxoplasma gondii lysates treated with enhancer 1 (ENH1) |
Description | Previously, we characterized a chemical modulator, ENH1, that exhibited potent antiparasitic properties against apicomplexans and induced calcium oscillations, but the target eluded identification. To determine the target of ENH1, we employed thermal proteome profiling in parasites. We treated extracellular parasites with vehicle (DMSO) or ENH1 for 15 minutes at 37°C, under conditions previously determined to induce calcium fluxes in T. gondii.8 Parasites were then heated at 10 different temperatures to induce thermal denaturation. Following lysis and high-speed centrifugation, soluble protein was isolated and analyzed by mass spectrometry to generate thermal stability profiles from each condition across two biological replicates. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-08 |
AnnouncementXML | Submission_2024-10-08_11:12:04.556.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD018810 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Alice Herneisen |
SpeciesList | scientific name: Toxoplasma gondii GT1; NCBI TaxID: 507601; |
ModificationList | Biotin:Sigma-B1267; TMT6plex-126 reporter+balance reagent acylated residue; Phospho; Methylthio; methylthiolated residue; Label:13C(6)15N(4); TMT6plex; phosphorylated residue; Oxidation; isotope labeled residue; Acetyl; monohydroxylated residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-04-26 14:52:15 | ID requested | |
⏵ 1 | 2024-10-08 11:12:09 | announced |
Publication List
Herneisen AL, Sidik SM, Markus BM, Drewry DH, Zuercher WJ, Lourido S, Using Thermal Proteome Profiling. ACS Chem Biol, 15(7):1801-1807(2020) [pubmed] |
10.6019/PXD018810; |
10.1021/acschembio.0c00369; |
Keyword List
submitter keyword: thermal proteome profiling,Toxoplasma gondii, CETSA |
Contact List
Sebastian Lourido | |
---|---|
contact affiliation | Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Biology, Cambridge, MA, USA |
contact email | lourido@wi.mit.edu |
lab head | |
Alice Herneisen | |
contact affiliation | Whitehead Institute for Biomedical Research, Cambridge, MA, USA 02142 Department of Biology, Massachusetts Insitute of Technology |
contact email | alice@wi.mit.edu |
dataset submitter |
Full Dataset Link List
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PRIDE project URI |
Repository Record List
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