PXD018807

PXD018807 is an original dataset announced via ProteomeXchange.

Title	Copper detoxification machinery of the brain-eating amoeba Naegleria fowleri involves copper-translocating ATPase and the antioxidant system
Description	Copper is a trace metal that is necessary for all organisms but toxic when present in excess. Different mechanisms to avoid copper toxicity have been reported to date in pathogenic organisms such as Cryptococcus neoformans and Candida albicans. However, little if anything is known about pathogenic protozoans despite their importance in human and veterinary medicine. Naegleria fowleri is a free-living amoeba that occurs naturally in warm fresh water and can cause a rapid and deadly brain infection called primary amoebic meningoencephalitis (PAM). Here, we describe the mechanisms employed by N. fowleri to tolerate high copper concentrations, which include various strategies such as copper efflux mediated by a copper-translocating ATPase and upregulation of the expression of antioxidant enzymes and obscure hemerythrin-like and protoglobin-like proteins. The combination of different mechanisms efficiently protects the cell and ensures its high copper tolerance, which can be advantageous both in the natural environment and in the host. Nevertheless, we demonstrate that copper ionophores are potent antiamoebic agents; thus, copper metabolism may be considered a therapeutic target.
HostingRepository	PRIDE
AnnounceDate	2021-05-19
AnnouncementXML	Submission_2021-05-19_01:30:44.980.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Karel Harant
SpeciesList	scientific name: Naegleria fowleri; NCBI TaxID: 5763;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-26 14:42:34	ID requested
⏵ 1	2021-05-19 01:30:45	announced

Grechnikova M, Ž, en, í, š, kov, á K, Malych R, Mach J, Sutak R, Copper detoxification machinery of the brain-eating amoeba Naegleria fowleri involves copper-translocating ATPase and the antioxidant system. Int J Parasitol Drugs Drug Resist, 14():126-135(2020) [pubmed]

submitter keyword: copper, oxidative stress, copper transporting ATPase, Naegleria fowleri, ionophores, hemerythrin

Robert Sutak
contact affiliation	Robert Sutak, Department of Parasitology, Faculty of Science, Charles University, BIOCEV, Vestec, Czech Republic.
contact email	sutak@natur.cuni.cz
lab head
Karel Harant
contact affiliation	Charles University
contact email	harant@natur.cuni.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018807
     1. Label: PRIDE project
     2. Name: Copper detoxification machinery of the brain-eating amoeba Naegleria fowleri involves copper-translocating ATPase and the antioxidant system