PXD018794

PXD018794 is an original dataset announced via ProteomeXchange.

Title	Precise temporal regulation of post-transcriptional repressors is required for an orderly Drosophila maternal-to-zygotic transition
Description	In animal embryos the maternal-to-zygotic transition (MZT) hands developmental control from maternal to zygotic gene products. We show that the maternal proteome represents over half of the protein coding capacity of the Drosophila melanogaster genome and that 2% of this proteome is rapidly degraded during the MZT. Cleared proteins include the post-transcriptional repressors Cup, Trailer hitch (TRAL), Maternal expression at 31B (ME31B), and Smaug (SMG). While the ubiquitin-proteasome system is necessary for clearance of all four repressors, distinct E3 ligase complexes target them: the C-terminal to Lis1 Homology (CTLH) complex targets Cup, TRAL and ME31B for degradation early in the MZT; the Skp/Cullin/F-box-containing (SCF) complex targets SMG at the end of the MZT. Deleting the C-terminal 233 amino acids of SMG makes the protein immune to degradation. We show that artificially persistent SMG downregulates the zygotic re-expression of mRNAs whose maternal contribution is cleared by SMG. Thus, clearance of SMG permits an orderly MZT.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:27:37.205.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Ihling
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-24 05:12:31	ID requested
1	2021-09-08 19:46:01	announced
⏵ 2	2024-10-22 05:27:45	announced	2024-10-22: Updated project metadata.

10.1016/j.celrep.2020.107783;

Cao WX, Kabelitz S, Gupta M, Yeung E, Lin S, Rammelt C, Ihling C, Pekovic F, Low TCH, Siddiqui NU, Cheng MHK, Angers S, Smibert CA, W, ü, hr M, Wahle E, Lipshitz HD, Precise Temporal Regulation of Post-transcriptional Repressors Is Required for an Orderly Drosophila Maternal-to-Zygotic Transition. Cell Rep, 31(12):107783(2020) [pubmed]

submitter keyword: C-terminal to Lis1 Homology (CTLH) complex, Skp/Cullin/F-box-containing (SCF) complex, ubiquitin ligase,Smaug, RNA-binding protein

Elmar Wahle
contact affiliation	Institute of Biochemistry and Biotechnology Charles Tanford Protein Center Martin Luther University Halle-Wittenberg Germany
contact email	elmar.wahle@biochemtech.uni-halle.de
lab head
Christian Ihling
contact affiliation	MLU Halle, Inst. f. Pharmacy
contact email	christian.ihling@pharmazie.uni-halle.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD018794

PRIDE project URI

• PRIDE
  1. PXD018794
     1. Label: PRIDE project
     2. Name: Precise temporal regulation of post-transcriptional repressors is required for an orderly Drosophila maternal-to-zygotic transition