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PXD018790

PXD018790 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA Collision-induced Dissociation Cleavable Isobaric Tag for Peptide Fragment Ion-based Quantification in Proteomics
DescriptionFragment ion-based proteome quantification methods quantify peptides based on unique peptide fragment ions avoiding the issue of ratio distortion that is commonly observed for reporter ion-based quantification approaches. Herein, we present a novel approach that relies on a collision-induced dissociation (CID)-cleavable isobaric acetyl-isoleucine-proline-glycine (Ac-IPG) tag, which conserves the merits of quantifying peptides based on unique fragment ions while reducing the complexity of the b-ion series thus facilitating data processing. Multiplex labelling is based on selective N-terminal dimethylation followed by derivatizing the C-terminal Lys residue of LysC peptides with isobaric Ac-IPG tags with different isotope distributions on Pro-Gly and Ac-Ile. Upon fragmentation between Ile and Pro, the resulting y-ions with the neutral loss of Ac-Ile can be distinguished between the different labelling channels based on different numbers of isotope labels on the Pro-Gly part. The peak intensities of y-ions contain the information for relative quantification.
HostingRepositoryPRIDE
AnnounceDate2020-08-17
AnnouncementXMLSubmission_2020-08-16_21:57:34.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterXiaobo Tian
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-04-24 03:32:10ID requested
12020-08-16 21:57:35announced
Publication List
Tian X, de Vries MP, Permentier HP, Bischoff R, A Collision-Induced Dissociation Cleavable Isobaric Tag for Peptide Fragment Ion-Based Quantification in Proteomics. J Proteome Res, 19(9):3817-3824(2020) [pubmed]
Keyword List
submitter keyword: CID cleavable tag, fragment ion-based quantification, isotopic labelling, isobaric labelling, proteomics
Contact List
Rainer Bischoff
contact affiliationDepartment of Analytical Biochemistry Faculty of Science and Engineering University of Groningen
contact emailr.p.h.bischoff@rug.nl
lab head
Xiaobo Tian
contact affiliationUniversity of Groningen
contact emailtianxiaobo002@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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