PXD018790 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A Collision-induced Dissociation Cleavable Isobaric Tag for Peptide Fragment Ion-based Quantification in Proteomics |
Description | Fragment ion-based proteome quantification methods quantify peptides based on unique peptide fragment ions avoiding the issue of ratio distortion that is commonly observed for reporter ion-based quantification approaches. Herein, we present a novel approach that relies on a collision-induced dissociation (CID)-cleavable isobaric acetyl-isoleucine-proline-glycine (Ac-IPG) tag, which conserves the merits of quantifying peptides based on unique fragment ions while reducing the complexity of the b-ion series thus facilitating data processing. Multiplex labelling is based on selective N-terminal dimethylation followed by derivatizing the C-terminal Lys residue of LysC peptides with isobaric Ac-IPG tags with different isotope distributions on Pro-Gly and Ac-Ile. Upon fragmentation between Ile and Pro, the resulting y-ions with the neutral loss of Ac-Ile can be distinguished between the different labelling channels based on different numbers of isotope labels on the Pro-Gly part. The peak intensities of y-ions contain the information for relative quantification. |
HostingRepository | PRIDE |
AnnounceDate | 2020-08-17 |
AnnouncementXML | Submission_2020-08-16_21:57:34.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Xiaobo Tian |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-04-24 03:32:10 | ID requested | |
⏵ 1 | 2020-08-16 21:57:35 | announced | |
Publication List
Tian X, de Vries MP, Permentier HP, Bischoff R, A Collision-Induced Dissociation Cleavable Isobaric Tag for Peptide Fragment Ion-Based Quantification in Proteomics. J Proteome Res, 19(9):3817-3824(2020) [pubmed] |
Keyword List
submitter keyword: CID cleavable tag, fragment ion-based quantification, isotopic labelling, isobaric labelling, proteomics |
Contact List
Rainer Bischoff |
contact affiliation | Department of Analytical Biochemistry Faculty of Science and Engineering University of Groningen |
contact email | r.p.h.bischoff@rug.nl |
lab head | |
Xiaobo Tian |
contact affiliation | University of Groningen |
contact email | tianxiaobo002@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018790
- Label: PRIDE project
- Name: A Collision-induced Dissociation Cleavable Isobaric Tag for Peptide Fragment Ion-based Quantification in Proteomics