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PXD018787

PXD018787 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of phasin PhaF interaction partners and proteomic analysis of polyhydroxyalkanoate granules in Pseudomonas putida
DescriptionPhasin PhaF is a multifunctional protein associated with the surface of polyhydroxyalkanoate (PHA) granules (carbonosomes) that contributes significantly to PHA biogenesis in pseudomonads. PhaF participates in PHA granule stabilization and segregation and its deletion has a notable impact on overall transcriptome, PHA accumulation and cell physiology, suggesting a more extensive function than simply being a simply granule structural protein. We followed a systematic approach to detect potential interactions of PhaF with other components of the cell, which could pinpoint unexplored functions of PhaF in the regulation of PHA production. We determined the PhaF interactome in Pseudomonas putida KT2440 using pull-down-mass spectrometry (PD-MS), in which proteins interacting with PhaF were determined. We separately determined the PHA granule proteome (all proteins associated with PHA granules) in Pseudomonas putida KT2440 using two granule preparation methods.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:27:37.670.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRyan Kniewel
SpeciesList scientific name: Pseudomonas putida KT2440; NCBI TaxID: 160488;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-04-24 03:31:46ID requested
12021-09-08 19:33:00announced
22024-10-22 05:27:38announced2024-10-22: Updated project metadata.
Publication List
Tarazona NA, Hern, á, ndez-Arriaga AM, Kniewel R, Prieto MA, Phasin interactome reveals the interplay of PhaF with the polyhydroxyalkanoate transcriptional regulatory protein PhaD in Pseudomonas putida. Environ Microbiol, 22(9):3922-3936(2020) [pubmed]
10.1111/1462-2920.15175;
Keyword List
submitter keyword: PHA granules, Pseudomonas putida,Polyhydroxyalkanoate, carbonosomes, phasin interactome
Contact List
M. Auxiliadora Prieto
contact affiliationPolymer Biotechnology Lab Centro de Investigaciones Biologicas(CIB) Consejo Superior de Investigaciones Científicas (CSIC) C/ Ramiro de Maeztu, 9. 28040 Madrid, Spain
contact emailauxi@cib.csic.es
lab head
Ryan Kniewel
contact affiliationBiological Research Center-Spanish National Research Council (CIB-CSIC)
contact emailarkay0@gmail.com
dataset submitter
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Dataset FTP location
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