PXD018787

PXD018787 is an original dataset announced via ProteomeXchange.

Title	Identification of phasin PhaF interaction partners and proteomic analysis of polyhydroxyalkanoate granules in Pseudomonas putida
Description	Phasin PhaF is a multifunctional protein associated with the surface of polyhydroxyalkanoate (PHA) granules (carbonosomes) that contributes significantly to PHA biogenesis in pseudomonads. PhaF participates in PHA granule stabilization and segregation and its deletion has a notable impact on overall transcriptome, PHA accumulation and cell physiology, suggesting a more extensive function than simply being a simply granule structural protein. We followed a systematic approach to detect potential interactions of PhaF with other components of the cell, which could pinpoint unexplored functions of PhaF in the regulation of PHA production. We determined the PhaF interactome in Pseudomonas putida KT2440 using pull-down-mass spectrometry (PD-MS), in which proteins interacting with PhaF were determined. We separately determined the PHA granule proteome (all proteins associated with PHA granules) in Pseudomonas putida KT2440 using two granule preparation methods.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:27:37.670.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ryan Kniewel
SpeciesList	scientific name: Pseudomonas putida KT2440; NCBI TaxID: 160488;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-24 03:31:46	ID requested
1	2021-09-08 19:33:00	announced
⏵ 2	2024-10-22 05:27:38	announced	2024-10-22: Updated project metadata.

Tarazona NA, Hern, á, ndez-Arriaga AM, Kniewel R, Prieto MA, Phasin interactome reveals the interplay of PhaF with the polyhydroxyalkanoate transcriptional regulatory protein PhaD in Pseudomonas putida. Environ Microbiol, 22(9):3922-3936(2020) [pubmed]

10.1111/1462-2920.15175;

submitter keyword: PHA granules, Pseudomonas putida,Polyhydroxyalkanoate, carbonosomes, phasin interactome

M. Auxiliadora Prieto
contact affiliation	Polymer Biotechnology Lab Centro de Investigaciones Biologicas(CIB) Consejo Superior de Investigaciones Científicas (CSIC) C/ Ramiro de Maeztu, 9. 28040 Madrid, Spain
contact email	auxi@cib.csic.es
lab head
Ryan Kniewel
contact affiliation	Biological Research Center-Spanish National Research Council (CIB-CSIC)
contact email	arkay0@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD018787

PRIDE project URI

• PRIDE
  1. PXD018787
     1. Label: PRIDE project
     2. Name: Identification of phasin PhaF interaction partners and proteomic analysis of polyhydroxyalkanoate granules in Pseudomonas putida