PXD018787 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of phasin PhaF interaction partners and proteomic analysis of polyhydroxyalkanoate granules in Pseudomonas putida |
Description | Phasin PhaF is a multifunctional protein associated with the surface of polyhydroxyalkanoate (PHA) granules (carbonosomes) that contributes significantly to PHA biogenesis in pseudomonads. PhaF participates in PHA granule stabilization and segregation and its deletion has a notable impact on overall transcriptome, PHA accumulation and cell physiology, suggesting a more extensive function than simply being a simply granule structural protein. We followed a systematic approach to detect potential interactions of PhaF with other components of the cell, which could pinpoint unexplored functions of PhaF in the regulation of PHA production. We determined the PhaF interactome in Pseudomonas putida KT2440 using pull-down-mass spectrometry (PD-MS), in which proteins interacting with PhaF were determined. We separately determined the PHA granule proteome (all proteins associated with PHA granules) in Pseudomonas putida KT2440 using two granule preparation methods. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:27:37.670.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ryan Kniewel |
SpeciesList | scientific name: Pseudomonas putida KT2440; NCBI TaxID: 160488; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-04-24 03:31:46 | ID requested | |
1 | 2021-09-08 19:33:00 | announced | |
⏵ 2 | 2024-10-22 05:27:38 | announced | 2024-10-22: Updated project metadata. |
Publication List
Tarazona NA, Hern, á, ndez-Arriaga AM, Kniewel R, Prieto MA, Phasin interactome reveals the interplay of PhaF with the polyhydroxyalkanoate transcriptional regulatory protein PhaD in Pseudomonas putida. Environ Microbiol, 22(9):3922-3936(2020) [pubmed] |
10.1111/1462-2920.15175; |
Keyword List
submitter keyword: PHA granules, Pseudomonas putida,Polyhydroxyalkanoate, carbonosomes, phasin interactome |
Contact List
M. Auxiliadora Prieto |
contact affiliation | Polymer Biotechnology Lab Centro de Investigaciones Biologicas(CIB) Consejo Superior de Investigaciones Científicas (CSIC) C/ Ramiro de Maeztu, 9. 28040 Madrid, Spain |
contact email | auxi@cib.csic.es |
lab head | |
Ryan Kniewel |
contact affiliation | Biological Research Center-Spanish National Research Council (CIB-CSIC) |
contact email | arkay0@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018787
- Label: PRIDE project
- Name: Identification of phasin PhaF interaction partners and proteomic analysis of polyhydroxyalkanoate granules in Pseudomonas putida