PXD018786

PXD018786 is an original dataset announced via ProteomeXchange.

Title	Cross-linking mass spectrometry identifies interacting regions between VACV A46 and human MAL or MyD88
Description	Vaccinia virus immunomodulator A46 is a member of the poxviral Bcl-2-like protein family that inhibits the cellular innate immune response at the level of the TIR domain-containing TLR adaptor proteins MAL, MyD88, TRAM and TRIF. The mechanism of interaction of A46 with its targets has remained unclear. We used BS3 and EDC + sulfo-NHS to cross-link A46 to the TIR domains of MAL and MyD88 to help identify interacting residues and regions.
HostingRepository	PRIDE
AnnounceDate	2023-03-20
AnnouncementXML	Submission_2023-03-20_04:50:03.020.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	DorotheaAnrather
SpeciesList	scientific name: Vaccinia virus; NCBI TaxID: 10245;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-24 03:31:41	ID requested
1	2020-10-01 23:14:44	announced
⏵ 2	2023-03-20 04:50:03	announced	2023-03-20: Updated project metadata.

Azar DF, Haas M, Fedosyuk S, Rahaman MH, Hedger A, Kobe B, Skern T, Vaccinia Virus Immunomodulator A46: Destructive Interactions with MAL and MyD88 Shown by Negative-Stain Electron Microscopy. Structure, 28(12):1271-1287.e5(2020) [pubmed]

submitter keyword: TLR 4, QExactive HF-X, Bcl-2-like, TIR domain,Vaccinia, cross-linking MS

TimSkern
contact affiliation	Max Perutz Labs, Medical University of Vienna, Vienna BioCenter
contact email	timothy.skern@muv.ac.at
lab head
DorotheaAnrather
contact affiliation	Max Perutz Labs, Mass Spectrometry Facility, University of Vienna
contact email	dorothea.anrather@univie.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD018786

PRIDE project URI

• PRIDE
  1. PXD018786
     1. Label: PRIDE project
     2. Name: Cross-linking mass spectrometry identifies interacting regions between VACV A46 and human MAL or MyD88