PXD018779

PXD018779 is an original dataset announced via ProteomeXchange.

Title	Label-Free Proteomics Profiling of Nanovesicles Isolated from Cultured Salivary Glands Isolated from Partially-Fed Adult Female Ixodes scapularis
Description	Ticks are ectoparasites that have co-evolved with their hosts for millions of years. Unlike other blood-feeders, hard ticks take several days to complete a blood-meal, which makes them especially susceptible to the detection by host immune responses. To overcome this, ticks have developed a vast array of salivary effectors with immune modulatory properties. These effectors not only benefit tick survival but also influence pathogen transmission. We have characterized the extracellular vesicle protein cargo secreted within tick saliva. Extracellular vesicles were purified through density gradient from salivary gland cultures dissected from partially fed adult female Ixodes scapularis. Molecules present in the vesicles were then characterized using label-free proteomics. In this protein dataset, we have identified several tick immune modulatory proteins. This dataset demonstrates that arthropods secrete extracellular vesicles for the translocation of effectors during blood-feeding.
HostingRepository	PRIDE
AnnounceDate	2021-06-07
AnnouncementXML	Submission_2021-06-06_22:23:01.962.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD018779
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Cristina Clement
SpeciesList	scientific name: Ixodes scapularis; NCBI TaxID: 6945;
ModificationList	monomethylated residue; carbamoylated residue; amidated residue; 2-pyrrolidone-5-carboxylic acid (Gln); phosphorylated residue; monohydroxylated residue; 2-pyrrolidone-5-carboxylic acid (Pro); iodoacetic acid derivatized residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-24 01:18:47	ID requested
⏵ 1	2021-06-06 22:23:02	announced

Dataset with its publication pending

submitter keyword: Salivary Glands Ixodes Scapularis

label free proteomics

QExactive nano-LC/MS/MS

Joao Pedra
contact affiliation	Department of Microbiology and Immunology University of Maryland School of Medicine USA
contact email	JPedra@som.umaryland.edu
lab head
Cristina Clement
contact affiliation	Weill Cornell Medicine
contact email	ccc4002@med.cornell.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD018779

PRIDE project URI

• PRIDE
  1. PXD018779
     1. Label: PRIDE project
     2. Name: Label-Free Proteomics Profiling of Nanovesicles Isolated from Cultured Salivary Glands Isolated from Partially-Fed Adult Female Ixodes scapularis