PXD018775

PXD018775 is an original dataset announced via ProteomeXchange.

Title	Effect of Nitrosative Stress on the S-nitroso-Proteome of Paracoccidioides brasiliensis
Description	The fungi of Paracoccidioides spp. genus are the causative agents of paracoccidioidomycosis (PCM), a systemic mycosis endemic to Latin America. This fungus is considered a facultative intracellular pathogen that is able to survive and replicate inside macrophages. The survival of the fungus during infection depends on its adaptability to various conditions, such as nitrosative/oxidative stress produced by the host immune cells, particularly alveolar macrophages. Currently, there is little knowledge about the P. brasiliensis signaling pathways involved in the fungus evasion mechanism of the host defense response. However, it is known that some of these pathways are triggered by reactive oxygen species and reactive nitrogen species (ROS/RNS) produced by host cells. Considering that the effects of NO (nitric oxide) on pathogens are concentration dependent, such effects could alter the redox state of cysteine residues by influencing (activating or inhibiting) a variety of protein functions, notably S-nitrosylation, a highly important NO-dependent posttranslational modification that regulates cellular functions and signaling pathways. It has been demonstrated by our group that P. brasiliensis yeast cells proliferate when exposed to low NO concentrations. Thus, this work investigated the modulation profile of S-nitrosylated proteins of P. brasiliensis, as well as identifying S-nitrosylation sites after treatment with RNS. Through mass spectrometry analysis (LC-MS/MS) and label-free quantification, it was possible to identify 474 proteins in the S-nitrosylated proteome study. With this approach, we observed that proteins treated with NO at low concentrations presented a proliferative response pattern, with several proteins involved in cellular cycle regulation and growth being activated. These proteins appear to play important roles in fungal virulence. On the other hand, proteins stimulated by high NO concentrations exhibited a survival response pattern, which may help to elucidate RNS antifungal properties and identify potential molecular targets for the development of new drugs in the future
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:07:56.375.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marina Navarro
SpeciesList	scientific name: Paracoccidioides brasiliensis (strain Pb18); NCBI TaxID: 502780;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-24 01:18:21	ID requested
1	2020-06-27 16:57:13	announced
⏵ 2	2024-10-22 05:08:04	announced	2024-10-22: Updated project metadata.

10.3389/fmicb.2020.01184;

Navarro MV, Chaves AFA, Castilho DG, Casula I, Calado JCP, Concei, ç, ã, o PM, Iwai LK, de Castro BF, Batista WL, . Front Microbiol, 11():1184(2020) [pubmed]

submitter keyword: nitric oxide, S-nitrosylation,Paracoccidioides brasiliensis, S-nitroso-proteome

Wagner Luiz Batista
contact affiliation	Department of Pharmaceutical Sciences, Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Federal University of São Paulo, Diadema, SP, Brazil
contact email	batistawl@gmail.com
lab head
Marina Navarro
contact affiliation	Federal University of São Paulo
contact email	marinavnavarro@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD018775

PRIDE project URI

• PRIDE
  1. PXD018775
     1. Label: PRIDE project
     2. Name: Effect of Nitrosative Stress on the S-nitroso-Proteome of Paracoccidioides brasiliensis