PXD018774

PXD018774 is an original dataset announced via ProteomeXchange.

Title	Modulation of immune cell reactivity with cis-binding Siglec-9 agonists
Description	Primary inflammatory pathologies caused by phagocytes lead to numerous debilitating conditions, ranging from chronic pain to permanent blindness. Siglec-9 is an immunoinhibitory receptor expressed on many types of phagocytes and is a promising target for anti-inflammatory therapeutics. We developed a lipid-tethered glycopolypeptide that spontaneously inserts into cell membranes and specifically binds Siglec-9 in cis on the surface of cells. We demonstrate that when inserted in a cell membrane and cis-binding, but not as a soluble trans-binding agent, this glycopolypeptide and agonizes Siglec-9, inhibiting inflammatory activity in reporter systems, phagocytic cell lines, and primary human macrophages. Thus, membrane-tethered cis-agonists of Siglec-9 are a new modality for therapeutic suppression of immune cell reactivity.
HostingRepository	PRIDE
AnnounceDate	2021-01-14
AnnouncementXML	Submission_2021-01-14_00:36:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nicholas Riley
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-24 01:18:18	ID requested
⏵ 1	2021-01-14 00:36:34	announced

Delaveris CS, Chiu SH, Riley NM, Bertozzi CR, -binding Siglec agonists. Proc Natl Acad Sci U S A, 118(3):(2021) [pubmed]

submitter keyword: Phosphoproteomics, LC-MS/MS, TMT

Carolyn Bertozzi
contact affiliation	Department of Chemistry, Stanford University Howard Hughes Medical Institute, Stanford University
contact email	bertozzi@stanford.edu
lab head
Nicholas Riley
contact affiliation	Stanford University
contact email	riley.nicholasm@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018774
     1. Label: PRIDE project
     2. Name: Modulation of immune cell reactivity with cis-binding Siglec-9 agonists