PXD018772

PXD018772 is an original dataset announced via ProteomeXchange.

Title	Dissecting the molecular mechanisms of mother-to-egg immune protection in the mealworm beetle Tenebrio molitor
Description	In a number of species, individuals exposed to pathogens can mount an immune response and transmit this immunological experience to their offspring, thereby protecting them against persistent threats. Such vertical transfer of immunity, named trans-generational immune priming (TGIP), has been described in both vertebrates and invertebrates. Although increasingly studied during the last decade, the mechanisms underlying TGIP in invertebrates are still elusive, especially those protecting the earliest offspring life stage, i.e. the embryo developing in the egg. In the present study, we took the mealworm beetle Tenebrio molitor as a biological model, and combined different proteomic and transcriptomic approaches to determine whether mothers transfer a “signal” (such as fragments of infecting bacteria), mRNA and/or protein/peptide effectors to protect their eggs against two natural bacterial pathogens, namely the Gram-positive Bacillus thuringiensis (Bt) and the Gram-negative Serratia entomophila (Se). We demonstrated that eggs are mainly protected by an active direct transfer of a restricted number of immune proteins and of antimicrobial peptides. In contrast, the present data clearly reject the involvement of mRNA transfer while the transmission of a “signal”, if it happens, is marginal and only occurs within 24h after maternal exposure to bacteria. This work exemplifies the power of combining global multidisciplinary approaches to disentangle the different scenarios of a complex trait, providing a comprehensive characterization of TGIP mechanisms in T. molitor. It also paves the way for future alike studies focusing on TGIP in a wide range of invertebrates and vertebrates to identify additional candidates that could be specific to TGIP and to investigate whether the TGIP mechanisms found herein are specific or common to all insect species.
HostingRepository	PRIDE
AnnounceDate	2020-10-08
AnnouncementXML	Submission_2020-10-08_00:00:46.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sebastien Voisin
SpeciesList	scientific name: Tenebrio molitor; NCBI TaxID: 7067;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-24 01:17:59	ID requested
⏵ 1	2020-10-08 00:00:47	announced

Dataset with its publication pending

submitter keyword: Trans-generational immune priming

invertebrate immunity

host-pathogens interaction

molecular mechanisms

Omics

antimicrobial peptides

Philippe Bulet
contact affiliation	CR Université Grenoble Alpes, Institute for Advanced Biosciences, INSERM U1209, CNRS UMR5309, La Tronche, France. Plateforme BioPark d'Archamps, ArchParc, Saint Julien en Genevois, France
contact email	philippe.bulet@univ-grenoble-alpes.fr
lab head
Sebastien Voisin
contact affiliation	Plateforme BioPark of Archamps
contact email	sebastien.voisin@biopark-archamps.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018772
     1. Label: PRIDE project
     2. Name: Dissecting the molecular mechanisms of mother-to-egg immune protection in the mealworm beetle Tenebrio molitor