PXD018757 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphoproteomics identifies microglial Siglec-F inflammatory response during neurodegeneration |
Description | This study sought to explore phosphoproteome signaling dysregulation in different models of neurodegeneration. We collected phosphotyrosine, MAPK/CDK substrates, global phosphoproteome, and protein expression data from hippocampus and/or cortex tissue from each model. Tryptic peptides were multiplexd with TMT, enriched for phosphopeptides, and then identified and quantified by LC-MS/MS. We additionally quantified phosphotyrosine peptides from BV-2 cells expressing various Siglec receptor constructs. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-08 |
AnnouncementXML | Submission_2024-10-08_11:12:43.439.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD018757 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Nader Morshed |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; Phospho; TMT6plex; phosphorylated residue; Oxidation; monohydroxylated residue; Carbamidomethyl; iodoacetamide derivatized residue |
Instrument | Q Exactive; LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-04-23 01:03:18 | ID requested | |
⏵ 1 | 2024-10-08 11:12:43 | announced | |
Publication List
Keyword List
submitter keyword: Tau P301S, Siglec-F, Mouse, 5XFAD, BV-2, phosphotyrosine,Alzheimer's disease, CK-p25, LC-MS/MS |
Contact List
Forest Michael White |
contact affiliation | Department of Biological Engineering, Koch Institute of Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA |
contact email | fwhite@mit.edu |
lab head | |
Nader Morshed |
contact affiliation | Massachusetts Institute of Technology |
contact email | morshed@mit.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD018757 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018757
- Label: PRIDE project
- Name: Phosphoproteomics identifies microglial Siglec-F inflammatory response during neurodegeneration