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PXD018749

PXD018749 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe Mi-2 nucleosome remodeler and the Rpd3 histone deacetylase are involved in piRNA-guided heterochromatin formation
DescriptionIn eukaryotes, trimethylation of lysine 9 on histone H3 (H3K9) is associated with transcriptional silencing of transposable elements (TEs). In drosophila ovaries, this heterochromatic repressive mark is thought to be deposited by SetDB1 on TE genomic loci after the initial recognition of nascent transcripts by PIWI-interacting RNAs (piRNAs) loaded on the Piwi protein. Here, we show that the nucleosome remodeler Mi-2, in complex with its partner MEP-1, forms a subunit that is transiently associated, in a MEP-1 C-terminus-dependent manner, with known Piwi interactors, including a recently reported SUMO ligase Su(var)2-10. Together with the histone deacetylase Rpd3, this module is involved in the piRNA-dependent TE silencing, correlated with H3K9 deacetylation and trimethylation. Therefore. drosophila piRNA-mediated transcriptional silencing involves three epigenetic effectors, a remodeler, Mi-2, an eraser, Rpd3 and a writer, SetDB1, in addition to the Su(var)2-10 SUMO ligase.
HostingRepositoryPRIDE
AnnounceDate2020-06-03
AnnouncementXMLSubmission_2020-06-09_23:30:15.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD018749
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterseverine chambeyron
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListMethyl; Oxidation; Carbamidomethyl
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-04-23 00:47:28ID requested
12020-06-02 18:31:02announced
22020-06-09 23:30:16announced2020-06-10: Updated publication reference for PubMed record(s): 32499524.
Publication List
Mugat B, Nicot S, Varela-Chavez C, Jourdan C, Sato K, Basyuk E, Juge F, Siomi MC, P, é, lisson A, Chambeyron S, The Mi-2 nucleosome remodeler and the Rpd3 histone deacetylase are involved in piRNA-guided heterochromatin formation. Nat Commun, 11(1):2818(2020) [pubmed]
Keyword List
submitter keyword: Gtsf1, Mi-2, MEP-1, RPD3
Contact List
CHAMBEYRON SEVERINE
contact affiliationInstitut Of Human Genetics, CNRS, Univ. Montpellier, UMR 9002, France
contact emailSeverine.chambeyron@igh.cnrs.fr
lab head
severine chambeyron
contact affiliationCNRS-Université de Montepllier
contact emailseverine.chambeyron@igh.cnrs.fr
dataset submitter
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Dataset FTP location
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