PXD018747

PXD018747 is an original dataset announced via ProteomeXchange.

Title	Genetic and proteomic screens suggest that signaling by the mechanosensitive ion channel MSL10 is influenced by its association with a synaptotagmin complex
Description	Plants respond to mechanical stressors in a variety of ways, producing electrical and biochemical signals, altering gene expression profiles, and changing organellar organization and morphology. We used a combination of genetic and proteomic approaches to identify genes that modify signaling of MSL10, a mechanosensitive ion channel in Arabidopsis which promotes programmed cell death in response to the mechanical stress from osmotic cell swelling. Mass spectrometry was used to identify proteins that co-immunoprecipitate with MSL10-GFP or with MSL107xD-GFP, a phosphomimetic variant that cannot activate cell death signaling. Interestingly, proteomics identified many proteins that are found at ER-plasma membrane contact sites (EPCSs). Two of the most abundant proteins that co-precipitated with MSL10 are the EPCS proteins VAP27-1 and SYT1, both of which have been reported to relocalize into spherical membrane structures in response to mechanical stress, a phenomenon also observed with MSL10. SYT1 has previously been shown to promote resistance to mechanical stress. A suppressor screen of the gain-of-function msl10-3G allele yielded mutants with point mutations in synaptotagmin (SYT)5 and SYT7, proteins that form a functional complex with SYT1. Together, these results suggest that MSL10 interacts with SYT1, SYT5, and SYT7 at EPCSs and that its cell death signaling output is modulated by this interaction.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:57:31.001.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fionn McLoughlin
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-22 23:54:03	ID requested
1	2022-10-26 08:44:08	announced
⏵ 2	2023-11-14 07:57:33	announced	2023-11-14: Updated project metadata.

Codjoe JM, Richardson RA, McLoughlin F, Vierstra RD, Haswell ES, . Elife, 11():(2022) [pubmed]

submitter keyword: Arabidopsis, Interacting proteins,Mechanosensitive Channels

Elizabeth Haswell
contact affiliation	Department of Biology, Washington University in St. Louis, St. Louis, MO, USA
contact email	ehaswell@wustl.edu
lab head
Fionn McLoughlin
contact affiliation	Washington University in Saint Louis
contact email	fmcloughlin@wustl.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018747
     1. Label: PRIDE project
     2. Name: Genetic and proteomic screens suggest that signaling by the mechanosensitive ion channel MSL10 is influenced by its association with a synaptotagmin complex