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PXD018740

PXD018740 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDeconstructing a type III secretion system effector network unravels the inherent robustness and plasticity in pathogenesis and immunity
DescriptionMany Gram-negative pathogens use type III secretion systems to inject networks of effectors that subvert host cell processes. We modelled the robustness of such network using the mouse pathogen Citrobacter rodentium (encoding 31 effectors) through sequential deletions of effector genes, generating 100 distinct mutant combinations, which were tested in vivo. Counterintuitively, mutants lacking 19 unrelated effectors (CR14) or 10 immune-modulating effectors (CRi9) colonised and induced conserved molecular signatures of infection in intestinal epithelial cells. Specifically, CRi9 triggered heightened colonic secretion of TNF, IL-22, IL-17A and IFN-γ, while CR14 induced high-level secretion of IL-6 and GM-CSF with lower recruitment of myeloid cells. Yet, both CR14 and CRi9 induced sterile immunity. A machine learning model, trained on the mutant phenotypes and effector function, predicted an unprecedented combination of colonisation-failure effector mutations, which was validated experimentally. These results reveal the robustness and combinatorial plasticity of both the effector network and host immunity.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:37:20.430.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJames Wright
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Citrobacter rodentium; NCBI TaxID: 67825;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-04-22 15:21:42ID requested
12022-05-31 05:53:39announced
22024-10-22 05:37:20announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: MS-MS, TMT, mouse,Citrobacter rodentium
Contact List
Jyoti Choudhary
contact affiliationThe Institute of Cancer Research, London
contact emailjyoti.choudhary@icr.ac.uk
lab head
James Wright
contact affiliationThe Institute of Cancer Research
contact emailjames.wright@icr.ac.uk
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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