PXD018737 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Formaldehyde derivatization, a Side Reaction During Filter-Aided Sample Preparation |
Description | The filter-aided sample preparation (FASP) method has been commonly used for proteomic sample preparation due to its high efficiency and convenience. Herein, we report an overlooked side reaction during FASP method, resulting in a mass increment of 12 Da. We confirmed that the side reaction is caused by formaldehyde released from the spin filter and devel-oped a pretreatment step to reduce the side reaction. We further examined spin filters from different vendors and offered suggestions for different experimental purpose. Our findings would benefit the deep screening of labeling proteomics analy-sis and offer a new direction to improve performance of filter devices used for different biological and proteomics studies. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:11:10.592.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Zhen Wu |
SpeciesList | scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-04-22 07:35:51 | ID requested | |
1 | 2020-08-20 03:37:16 | announced | |
⏵ 2 | 2024-10-22 05:11:11 | announced | 2024-10-22: Updated project metadata. |
Publication List
Tang L, Wu Z, Wang J, Zhang X, Formaldehyde Derivatization: An Unexpected Side Reaction During Filter-Aided Sample Preparation. Anal Chem, 92(18):12120-12125(2020) [pubmed] |
10.1021/acs.analchem.0c01981; |
Keyword List
submitter keyword: Filter-Aided Sample Preparation,Side Reaction,Formaldehyde derivatization |
Contact List
Xumin Zhang |
contact affiliation | State Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University, Shanghai, China |
contact email | xumin_zhang@fudan.edu.cn |
lab head | |
Zhen Wu |
contact affiliation | State Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University |
contact email | wuz16@fudan.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD018737
- Label: PRIDE project
- Name: Formaldehyde derivatization, a Side Reaction During Filter-Aided Sample Preparation