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PXD018737

PXD018737 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFormaldehyde derivatization, a Side Reaction During Filter-Aided Sample Preparation
DescriptionThe filter-aided sample preparation (FASP) method has been commonly used for proteomic sample preparation due to its high efficiency and convenience. Herein, we report an overlooked side reaction during FASP method, resulting in a mass increment of 12 Da. We confirmed that the side reaction is caused by formaldehyde released from the spin filter and devel-oped a pretreatment step to reduce the side reaction. We further examined spin filters from different vendors and offered suggestions for different experimental purpose. Our findings would benefit the deep screening of labeling proteomics analy-sis and offer a new direction to improve performance of filter devices used for different biological and proteomics studies.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:11:10.592.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterZhen Wu
SpeciesList scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-04-22 07:35:51ID requested
12020-08-20 03:37:16announced
22024-10-22 05:11:11announced2024-10-22: Updated project metadata.
Publication List
Tang L, Wu Z, Wang J, Zhang X, Formaldehyde Derivatization: An Unexpected Side Reaction During Filter-Aided Sample Preparation. Anal Chem, 92(18):12120-12125(2020) [pubmed]
10.1021/acs.analchem.0c01981;
Keyword List
submitter keyword: Filter-Aided Sample Preparation,Side Reaction,Formaldehyde derivatization
Contact List
Xumin Zhang
contact affiliationState Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University, Shanghai, China
contact emailxumin_zhang@fudan.edu.cn
lab head
Zhen Wu
contact affiliationState Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University
contact emailwuz16@fudan.edu.cn
dataset submitter
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Dataset FTP location
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Repository Record List
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