PXD018737

PXD018737 is an original dataset announced via ProteomeXchange.

Title	Formaldehyde derivatization, a Side Reaction During Filter-Aided Sample Preparation
Description	The filter-aided sample preparation (FASP) method has been commonly used for proteomic sample preparation due to its high efficiency and convenience. Herein, we report an overlooked side reaction during FASP method, resulting in a mass increment of 12 Da. We confirmed that the side reaction is caused by formaldehyde released from the spin filter and devel-oped a pretreatment step to reduce the side reaction. We further examined spin filters from different vendors and offered suggestions for different experimental purpose. Our findings would benefit the deep screening of labeling proteomics analy-sis and offer a new direction to improve performance of filter devices used for different biological and proteomics studies.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:11:10.592.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhen Wu
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-22 07:35:51	ID requested
1	2020-08-20 03:37:16	announced
⏵ 2	2024-10-22 05:11:11	announced	2024-10-22: Updated project metadata.

Tang L, Wu Z, Wang J, Zhang X, Formaldehyde Derivatization: An Unexpected Side Reaction During Filter-Aided Sample Preparation. Anal Chem, 92(18):12120-12125(2020) [pubmed]

10.1021/acs.analchem.0c01981;

submitter keyword: Filter-Aided Sample Preparation,Side Reaction,Formaldehyde derivatization

Xumin Zhang
contact affiliation	State Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University, Shanghai, China
contact email	xumin_zhang@fudan.edu.cn
lab head
Zhen Wu
contact affiliation	State Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University
contact email	wuz16@fudan.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/08/PXD018737

PRIDE project URI

• PRIDE
  1. PXD018737
     1. Label: PRIDE project
     2. Name: Formaldehyde derivatization, a Side Reaction During Filter-Aided Sample Preparation