PXD018734

PXD018734 is an original dataset announced via ProteomeXchange.

Title	Identification of VE-cadherin associated proteins regulated through the lncRNA LASSIE
Description	The shear stress-regulated lncRNA LASSIE interacts with cytoskeletal proteins as shown by RNA-antisense purification and subsequent mass spectrometry analysis using an anti-LASSIE and a non-targeting 3’ Desthiobiotin-TEG labeled 2’ O-Me-RNA. As this analysis resulted in high protein binding to the non-targeting control oligonucleotide, the RNA antisense purification was repeated in HUVECs treated with control and anti-LASSIE siRNA. Mass spectrometry analysis confirmed binding of LASSIE to the Intermediate filament protein nestin, as nestin enrichment was decreased in the absence of LASSIE. Additional functional analyses demonstrate a role of LASSIE in shear stress sensing and barrier function in endothelial cells, by stabilizing endothelial cell junctions through connection to the cytoskeleton.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:27:41.709.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilka Wittig
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-22 06:12:20	ID requested
1	2021-09-08 19:46:23	announced
⏵ 2	2024-10-22 05:27:42	announced	2024-10-22: Updated project metadata.

10.1038/s42003-020-0987-0;

Stanicek L, Lozano-Vidal N, Bink DI, Hooglugt A, Yao W, Wittig I, van Rijssel J, van Buul JD, van Bergen A, Klems A, Ramms AS, Le Noble F, Hofmann P, Szulcek R, Wang S, Offermanns S, Ercanoglu MS, Kwon HB, Stainier D, Huveneers S, Kurian L, Dimmeler S, Boon RA, Long non-coding RNA LASSIE regulates shear stress sensing and endothelial barrier function. Commun Biol, 3(1):265(2020) [pubmed]

submitter keyword: RNA/protein interaction

Reinier Boon
contact affiliation	Institute for Cardiovascular Regeneration, Centre for Molecular Medicine, Goethe University Frankfurt am Main, Germany
contact email	Boon@med.uni-frankfurt.de
lab head
Ilka Wittig
contact affiliation	Functional Proteomics, Goethe University, Frankfurt am Main , Germany
contact email	wittig@med.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD018734

PRIDE project URI

• PRIDE
  1. PXD018734
     1. Label: PRIDE project
     2. Name: Identification of VE-cadherin associated proteins regulated through the lncRNA LASSIE