PXD018733

PXD018733 is an original dataset announced via ProteomeXchange.

Title	The translation release factor methyltransferase Mtq2-Trm112 is required for ribosome biogenesis
Description	The Mtq2-Trm112 methyltransferase is known to modify the translational eukaryotic release factor eRF1 on the glutamine side chain of a universally conserved GGQ motif, which is essential to translation termination. The exact role of eRF1 methylation remains unknown. As the deletion of MTQ2 leads to severe growth impairment in S. cerevisiae, we have investigated its role further. Here we report an unexpected function of Mtq2 in ribosome biogenesis. We reveal that Mtq2 is part of nuclear precursor ribosomes to 60S subunits, and we demonstrate that its catalytic activity is required for the stable production of mature 5.8S and 25S rRNAs and for pre-60S export. Consistently, in the absence of Mtq2 function pre-60S subunits enriched in the export factors Nmd3 and Arx1 accumulate in the nucleus. We conclude that, in addition to its role as a translation terminator modification enzyme, Mtq2 is a ribosome assembly factor which is important for late-stage large ribosomal subunit formation.
HostingRepository	PRIDE
AnnounceDate	2020-10-12
AnnouncementXML	Submission_2020-11-16_16:14:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nicolas Pythoud
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	S-carboxamidoethyl-L-cysteine; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-22 05:18:19	ID requested
1	2020-10-12 06:40:41	announced
⏵ 2	2020-11-16 16:14:26	announced	2020-11-17: Updated publication reference for PubMed record(s): 33166396.

Lacoux C, Wacheul L, Saraf K, Pythoud N, Huvelle E, Figaro S, Graille M, Carapito C, Lafontaine DLJ, Heurgu, é, -Hamard V, The catalytic activity of the translation termination factor methyltransferase Mtq2-Trm112 complex is required for large ribosomal subunit biogenesis. Nucleic Acids Res, 48(21):12310-12325(2020) [pubmed]

submitter keyword: ribosome, translation, RNA processing, methyltransferase

Christine Carapito
contact affiliation	IPHC (LSMBO - Laboratoire de Spectrométrie de Masse Bio-Organique), UMR 7178, Université de Strasbourg, CNRS
contact email	ccarapito@unistra.fr
lab head
Nicolas Pythoud
contact affiliation	University of Strasbourg
contact email	nicolas.pythoud@etu.unistra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018733
     1. Label: PRIDE project
     2. Name: The translation release factor methyltransferase Mtq2-Trm112 is required for ribosome biogenesis