PXD018725

PXD018725 is an original dataset announced via ProteomeXchange.

Title	VE-cadherin associated proteins in the absence of lncRNA LASSIE
Description	The shear stress-regulated lncRNA LASSIE interacts with junctional proteins (e.g. PECAM-1, which interacts with VE-cadherin) and influences endothelial barrier function. Here we characterize the remodeling of the VE-Cadherin complex by the lncRNA LASSIE. LASSIE silenced HUVECs were subjected to co-immunoprecipitation using an anti-VE-cadherin antibody. Differentially associated proteins were identified by Mass spectrometry. This analysis revealed a significantly decreased association of cytoskeleton-linked proteins with VE-cadherin after silencing of LASSIE. Functional assays confirmed this result and characterized LASSIE as a stabilizer of junctional complexes in endothelial cells, important for normal shear stress sensing and barrier function.
HostingRepository	PRIDE
AnnounceDate	2022-02-15
AnnouncementXML	Submission_2022-02-15_10:55:57.483.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilka Wittig
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-21 23:02:41	ID requested
⏵ 1	2022-02-15 10:55:58	announced

Stanicek L, Lozano-Vidal N, Bink DI, Hooglugt A, Yao W, Wittig I, van Rijssel J, van Buul JD, van Bergen A, Klems A, Ramms AS, Le Noble F, Hofmann P, Szulcek R, Wang S, Offermanns S, Ercanoglu MS, Kwon HB, Stainier D, Huveneers S, Kurian L, Dimmeler S, Boon RA, Long non-coding RNA LASSIE regulates shear stress sensing and endothelial barrier function. Commun Biol, 3(1):265(2020) [pubmed]

submitter keyword: RNA/protein interaction

Reinier Boon
contact affiliation	Institute for Cardiovascular Regeneration, Centre for Molecular Medicine, Goethe University Frankfurt am Main, Germany
contact email	Boon@med.uni-frankfurt.de
lab head
Ilka Wittig
contact affiliation	Functional Proteomics, Goethe University, Frankfurt am Main , Germany
contact email	wittig@med.uni-frankfurt.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD018725

PRIDE project URI

• PRIDE
  1. PXD018725
     1. Label: PRIDE project
     2. Name: VE-cadherin associated proteins in the absence of lncRNA LASSIE