PXD018722

PXD018722 is an original dataset announced via ProteomeXchange.

Title	Signature activities of 20S proteasome reveal degradation of intact ubiquitin-conjugates in hypoxia
Description	Careful removal of unwanted proteins is necessary for cell survival. The primary constitutive intracellular protease is the 26S proteasome complex, very often found in equilibrium with its catalytic core particle – the 20S subcomplex. Protein degradation by the former is tightly regulated due to prior selection of substrates by ubiquitination, whereas the latter is most likely confined to substrates with an inherent unstructured stretch. Understanding the interplay between 26S and 20S proteasomes is challenging due to their common catalytic sites. By using MS analyses, we define the 20S and 26S substrate preferences, and ascribe a unique property to 20S in degrading ubiquitin. High-end mass-spectroscopy of intracellular peptidome defines signature activities of 20S and finds their contribution to proteostasis under severe hypoxia and in human failing heart.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:29:56.483.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oded Kleifeld
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-21 15:20:06	ID requested
1	2021-11-02 05:06:01	announced
⏵ 2	2024-10-22 05:29:56	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: 26S, 20S, peptides,Proteasome, Hypoxia

Oded Kleifeld
contact affiliation	Faculty of Biology Technion-Israel Institute of Technology Rooms 433-4, Biology Building Technion City, Haifa 3200003, Israel
contact email	okleifeld@technion.ac.il
lab head
Oded Kleifeld
contact affiliation	Technion
contact email	okleifeld@technion.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD018722

PRIDE project URI

• PRIDE
  1. PXD018722
     1. Label: PRIDE project
     2. Name: Signature activities of 20S proteasome reveal degradation of intact ubiquitin-conjugates in hypoxia