PXD018710

PXD018710 is an original dataset announced via ProteomeXchange.

Title	Dynamic Palmitoylation Events Following T-Cell Receptor Signaling
Description	Palmitoylation is the reversible addition of palmitate to cysteine via a thioester linkage. The reversible nature of this modification makes it a prime candidate as a mechanism for regulating signal transduction in T-cell receptor signaling. Following stimulation of the T-cell receptor we find a number of proteins are newly palmitoylated, including those involved in vesicle-mediated transport and Ras signal transduction. Among these stimulation-dependent palmitoylation targets are the v-SNARE VAMP7, important for docking of vesicular LAT during TCR signaling, and the largely undescribed palmitoyl acyltransferase DHHC18 that is expressed in two isoforms in T cells. Using our newly developed On-Plate Palmitoylation Assay (OPPA), we show DHHC18 is capable of palmitoylating VAMP7 at Cys183. Cellular imaging shows that the palmitoylation-deficient protein fails to be retained at the Golgi. This dataset includes the experiments previously reported in: Morrison et al. Quantitative analysis of the human T cell palmitome. Sci Rep. 5, Article number: 11598 (2015)
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-08_18:52:45.041.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Miguel Alvaro
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-21 05:34:22	ID requested
⏵ 1	2021-09-08 18:52:45	announced

Morrison E, Wegner T, Zucchetti AE, Á, lvaro-Benito M, Zheng A, Kliche S, Krause E, Br, ü, gger B, Hivroz C, Freund C, Dynamic palmitoylation events following T-cell receptor signaling. Commun Biol, 3(1):368(2020) [pubmed]

submitter keyword: Palmitoylation, S-acylation, TCR Signaling, T-Cell Activation, Palmitome, In vitro palmitoylation, VAMP7, Ti-VAMP, LAT, DHHC PATs, DHHC18, DHHC20

Christian Freund
contact affiliation	Freie Universität Berlin, Institut für Chemie und Biochemie, Thielallee 63, Berlin, 14195, Germany
contact email	chfreund@zedat.fu-berlin.de
lab head
Miguel Alvaro
contact affiliation	FU Berlin
contact email	malvaro@zedat.fu-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD018710

PRIDE project URI

• PRIDE
  1. PXD018710
     1. Label: PRIDE project
     2. Name: Dynamic Palmitoylation Events Following T-Cell Receptor Signaling